Key Product Details

Species Reactivity

Multi-Species

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2259C
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Product Specifications

Immunogen

Full length Cetuximab

Specificity

Detects Cetuximab in Western blots and direct ELISAs. No cross-reactivity with Adalimumab, Rituximab, Trastuzumab, Brentuximab, Gemtuzumab, Ibritumomab, Tositumomab, Solanezumab, Teprotumumab, and Aducanumab is observed.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Anti- Cetuximab (Anti-Idiotype) PE‑conjugated Antibody

Detection of Anti-EGFR (Cetuximab) on A431 cells by Flow Cytometry.

A431 cells were stained with Human Anti-Human EGFR (Research Grade Cetuximab Biosimilar) Monoclonal Antibody (MAB9577, filled histogram) or isotype control antibody (1-001-A, open histogram), followed by Rabbit Anti-Cetuximab (Anti-Idiotype) PE monoclonal antibody (Catalog # FAB9626P). View our protocol for Staining Membrane-associated Proteins.

Applications for Anti- Cetuximab (Anti-Idiotype) PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: A431 human epithelial carcinoma cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Cetuximab

The anti-Cetuximab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Cetuximab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.

Additional Cetuximab Products

Product Documents for Anti- Cetuximab (Anti-Idiotype) PE‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Anti- Cetuximab (Anti-Idiotype) PE‑conjugated Antibody

For research use only

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FAQs

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