Normal Human IgG Control

Catalog # Availability Size / Price Qty
1-001-A
Product Details
Citations (25)
FAQs
Reviews (4)

Normal Human IgG Control Summary

Source
Polyclonal Human IgG
Purification
Protein A or G purified
Immunogen
Normal human plasma (Donor plasma tested and found negative for HBsAg, HIV-1, HIV-2 and HCV antibodies by FDA approved tests)
Formulation
10 mM Sodium Phosphate, 0.85% (w/v) Sodium Chloride, pH 7.2 (PBS) with 5% trehalose and 0.05% (w/v) Sodium Azide
Label
Unconjugated

Applications

Recommended Concentration
Sample
Control
Please refer to the PDF datasheet for specifications.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute with sterile PBS. If 0.5 mL of PBS is used, the antibody concentration will be 2 mg/mL.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG

Product Datasheets

Citations for Normal Human IgG Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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  1. Immunoglobulin G responses against falciparum malaria specific antigens are higher in children with homozygous sickle cell trait than those with normal hemoglobin
    Authors: GM Bwire, M Majigo, R Makalla, L Nkinda, A Mawazo, M Mizinduko, J Makani
    BMC Immunol., 2019;20(1):12.
    Species: Human
    Sample Types: Protein
    Applications: ELISA Standard
  2. ITGA2 as a potential nanotherapeutic target for glioblastoma
    Authors: P Guo, A Moses-Gard, J Huang, ER Smith, MA Moses
    Sci Rep, 2019;9(1):6195.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  3. Calpain-mediated tau fragmentation is altered in Alzheimer's disease progression
    Authors: HH Chen, P Liu, P Auger, SH Lee, O Adolfsson, L Rey-Bellet, J Lafrance-V, BA Friedman, M Pihlgren, A Muhs, A Pfeifer, J Ernst, G Ayalon, KR Wildsmith, TG Beach, MP van der Br
    Sci Rep, 2018;8(1):16725.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Immunoprecipitation
  4. Comprehensive cross-clade characterization of antibody-mediated recognition, complement-mediated lysis and cell-mediated cytotoxicity of HIV-1 envelope specific antibodies towards the eradication of the HIV-1 reservoir
    Authors: S Mujib, J Liu, AKMN Rahman, JA Schwartz, P Bonner, FY Yue, MA Ostrowski
    J. Virol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Hypoxia-induced activin A diminishes endothelial cell vasculogenic activity
    Authors: S Merfeld-Cl, H Lu, X Wu, KL March, DO Traktuev
    J. Cell. Mol. Med., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
  6. HCV RNA Activates APCs via TLR7/TLR8 While Virus Selectively Stimulates Macrophages Without Inducing Antiviral Responses
    Sci Rep, 2016;6(0):29447.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  7. Glycoprotein nonmetastatic melanoma protein B extracellular fragment shows neuroprotective effects and activates the PI3K/Akt and MEK/ERK pathways via the Na+/K+-ATPase.
    Authors: Yoko Ono, Kazuhiro Tsuruma, Masafumi Takata, Masamitsu Shimazawa, Hideaki Hara
    Scientific Reports, 2016;0(0):2045-2322.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Control
  8. Targeting ADAM-17 with an inhibitory monoclonal antibody has antitumour effects in triple-negative breast cancer cells.
    Authors: Caiazza F, McGowan P, Mullooly M, Murray A, Synnott N, O'Donovan N, Flanagan L, Tape C, Murphy G, Crown J, Duffy M
    Br J Cancer, 2015;112(12):1895-903.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  9. Active immunization with Tocilizumab mimotopes induces specific immune responses.
    Authors: Yang L, Xing R, Li C, Liu Y, Sun L, Liu X, Wang Y
    BMC Biotechnol, 2015;15(0):46.
    Species: Bacteria - E. Coli
    Sample Types: Virus
    Applications: Isotype Control
  10. Hypoxia down-regulates expression of secretory leukocyte protease inhibitor in bronchial epithelial cells via TGF-beta1.
    Authors: Pahlman L, Jogi A, Gram M, Mori M, Egesten A
    BMC Pulm Med, 2015;15(0):19.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Induction and activation of human Th17 by targeting antigens to dendritic cells via dectin-1.
    Authors: Duluc D, Joo H, Ni L, Yin W, Upchurch K, Li D, Xue Y, Klucar P, Zurawski S, Zurawski G, Oh S
    J Immunol, 2014;192(12):5776-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  12. The aryl hydrocarbon receptor is functionally upregulated early in the course ofvhuman T-cell activation.
    Authors: Prigent L, Robineau M, Jouneau S, Morzadec C, Louarn L, Vernhet L, Fardel O, Sparfel L
    Eur J Immunol, 2014;44(5):1330-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Pathogenic strains of Acanthamoeba are recognized by TLR4 and initiated inflammatory responses in the cornea.
    Authors: Alizadeh H, Tripathi T, Abdi M, Smith A
    PLoS ONE, 2014;9(3):e92375.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  14. Generation of neuronal progenitor cells in response to tumors in the human brain.
    Authors: Macas J, Ku M, Nern C, Xu Y, Buhler H, Remke M, Synowitz M, Franz K, Seifert V, Plate K, Kettenmann H, Glass R, Momma S
    Stem Cells, 2014;32(1):244-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  15. B7-H1-expressing antigen-presenting cells mediate polarization of protumorigenic Th22 subsets.
    Authors: Kuang D, Xiao X, Zhao Q, Chen M, Li X, Liu R, Wei Y, Ouyang F, Chen D, Wu Y, Lao X, Deng H, Zheng L
    J Clin Invest, 2014;124(10):4657-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  16. RepSox slows decay of CD34+ acute myeloid leukemia cells and decreases T cell immunoglobulin mucin-3 expression.
    Authors: Jajosky A, Coad J, Vos J, Martin K, Senft J, Wenger S, Gibson L
    Stem Cells Transl Med, 2014;3(7):836-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  17. A novel murine cytomegalovirus vaccine vector protects against Mycobacterium tuberculosis.
    Authors: Beverley P, Ruzsics Z, Hey A, Hutchings C, Boos S, Bolinger B, Marchi E, O'Hara G, Klenerman P, Koszinowski U, Tchilian E
    J Immunol, 2014;193(5):2306-16.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  18. Sjogren's syndrome pathological neovascularization is regulated by VEGF-A-stimulated TACE-dependent crosstalk between VEGFR2 and NF-kappaB.
    Authors: Sisto M, Lisi S, Lofrumento DD, D'Amore M, Frassanito MA, Ribatti D
    Genes Immun., 2012;13(5):411-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Anti-Tumour Effects of a Specific Anti-ADAM17 Antibody in an Ovarian Cancer Model In Vivo.
    Authors: Richards FM, Tape CJ, Jodrell DI, Murphy G
    PLoS ONE, 2012;7(7):e40597.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  20. ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes.
    Authors: Wilhelmsen K, Mesa K, Lucero J, Xu F, Hellman J
    J Biol Chem, 2012;287(32):26478-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking
  21. Inhibition of Cell Proliferation by an Anti-EGFR Aptamer.
    Authors: Li N, Nguyen HH, Byrom M, Ellington AD
    PLoS ONE, 2011;6(6):e20299.
    Species: Human
    Sample Types: RNA
    Applications: Control
  22. Tumor cell marker PVRL4 (nectin 4) is an epithelial cell receptor for measles virus.
    Authors: Noyce RS, Bondre DG, Ha MN
    PLoS Pathog., 2011;7(8):e1002240.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: Fc Blocking
  23. Suppression of TNF-alpha and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27.
    Authors: Kalliolias GD, Gordon RA, Ivashkiv LB
    J. Immunol., 2010;185(11):7047-56.
    Species: Human
    Sample Types: Whole Cells
  24. HER2 phosphorylation is maintained by a PKB negative feedback loop in response to anti-HER2 herceptin in breast cancer.
    Authors: Gijsen M, King P, Perera T, Parker PJ, Harris AL, Larijani B, Kong A
    PLoS Biol., 2010;8(12):e1000563.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  25. TSU68 prevents liver metastasis of colon cancer xenografts by modulating the premetastatic niche.
    Authors: Yamamoto M, Kikuchi H, Ohta M, Kawabata T, Hiramatsu Y, Kondo K, Baba M, Kamiya K, Tanaka T, Kitagawa M, Konno H
    Cancer Res., 2008;68(23):9754-62.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Control

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Reviews for Normal Human IgG Control

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Normal Human IgG Control
By Anonymous on 02/16/2020
Application: WB Sample Tested: 293T human embryonic kidney cell line Species: Human

IgG was used as negative control.


Normal Human IgG Control
By Anonymous on 02/04/2020
Application: Func Sample Tested: Bladder cancer tissue Species: Human

UM-UC3 bladder cancer cells were transiently transfected with a Notch-reporter Firefly luciferase plasmid, in addition to an internal Renilla control. IgG was used as a negative stimulation control, whereas Jagged 1 was used to induce a Notch-specific response. Increasing concentrations of the Gamma-secretase inhibitor DAPT resulted in a dose-dependent decrease in Notch signal.


Normal Human IgG Control
By Anonymous on 09/22/2019
Application: WB Sample Tested: Recombinant protein Species: Human

Normal Human IgG Control
By Anonymous on 10/27/2018
Application: Flow Sample Tested: MBA-MB-123 human breast cancer cell line Species: Human

Used at 10 ug/mL works perfectly as a human Isotype control for flow cytometry. There is no background binding in live cells that do not express Fc receptor. The attached image shows MFI with and without the isotype control indicating identical signal.