Normal Human IgG Control

Catalog # Availability Size / Price Qty
1-001-A
Detection of Human IgG by Western Blot
1 Image
Product Details
Citations (56)
FAQs
Reviews (4)

Normal Human IgG Control Summary

Source
Polyclonal Human IgG
Purification
Protein A or G purified
Immunogen
Normal human plasma (Donor plasma tested and found negative for HBsAg, HIV-1, HIV-2 and HCV antibodies by FDA approved tests)
Formulation
10 mM Sodium Phosphate, 0.85% (w/v) Sodium Chloride, pH 7.2 (PBS) with 5% trehalose and 0.05% (w/v) Sodium Azide
Label
Unconjugated

Applications

Recommended Concentration
Sample
Control
Please refer to the PDF datasheet for specifications.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IgG by Western Blot View Larger

Detection of Human IgG by Western Blot Activated ADSCs trigger the proliferation and invasion of lung cancer cells by regulating MMP2/9 expression and EMT. a The effects of activated ADSCs on lung cancer cell proliferation were evaluated using the CCK-8 assay. Four lung cancer cell lines were cultured in the presence of ADSC-CM or aADSC-CM, and the optical density at 450 nm was analysed. The cancer cells cultured alone in normal growth medium were used as negative controls. Data from three separate experiments are shown. b The number of lung cancer cells that migrated through 8-μm Transwell membrane pores was counted to determine the changes in the invasive capabilities in response to CM from epidural ADSCs or aADSCs. c MMP2/9, E-cadherin and vimentin expression levels in four lung cancer cell lines treated with ADSC-CM or aADSC-CM were examined by western blotting. Lung cancer cells cultured in an untreated medium served as controls. d Lung cancer cells were treated with neutralizing antibodies against IL-6, isotype controls and aADSC-CM. Lung cancer cell invasion was analysed using a Transwell assay. Lung cancer cells cultured with untreated medium served as negative controls. e Lung cancer cells were treated with neutralizing antibodies against IL-6, isotype controls and aADSC-CM. MMP2/9, E-cadherin and vimentin expression levels in four lung cancer cell lines were analysed using western blotting. Lung cancer cells cultured with untreated medium served as negative controls. ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31196220), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

Reconstitution
Reconstitute with sterile PBS. If 0.5 mL of PBS is used, the antibody concentration will be 2 mg/mL.
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Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
♦ 12 months from date of receipt at -20 to -70 °C

♦ 6 months from date of receipt at 2 to 8 °C

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

Product Datasheets

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Product Specific Notices

Contains sodium azide.  Sodium azide may react with lead and copper plumbing to form explosive metal azides. Flush with large amounts of water during disposal.

Citations for Normal Human IgG Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

56 Citations: Showing 1 - 10
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  1. Inhibition of shedding of low-density lipoprotein receptor-related protein 1 reverses cartilage matrix degradation in osteoarthritis
    Authors: K Yamamoto, S Santamaria, KA Botkjaer, J Dudhia, L Troeberg, Y Itoh, G Murphy, H Nagase
    Arthritis & rheumatology (Hoboken, N.J.), 2017-04-28;0(0):.
  2. Involvement of CD40-CD40L and ICOS-ICOSL in the development of chronic rhinosinusitis by targeting eosinophils
    Authors: Aina Zhou, Chenxi Shi, Yuhui Fan, Yushuang Zheng, Jue Wang, Zhichen Liu et al.
    Frontiers in Immunology
  3. Blood-brain barrier penetration of non-replicating SARS-CoV-2 and S1 Variants of Concern induce neuroinflammation which is accentuated in a mouse model of Alzheimer's disease
    Authors: MA Erickson, AF Logsdon, EM Rhea, KM Hansen, SJ Holden, WA Banks, JL Smith, C German, SA Farr, JE Morley, RR Weaver, AJ Hirsch, A Kovac, E Kontsekova, KK Baumann, MA Omer, J Raber
    Brain, Behavior, and Immunity, 2023-01-20;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. Schwann cells promote the migration and invasion of colorectal cancer cells via the activated NF-kappa B/IL-8 axis in the tumor microenvironment
    Authors: Shuhai Chen, Mingyou Chen
    Frontiers in Oncology
  5. Dll4 Inhibition Promotes Graft Retention in Fat Grafting Enriched with Adipose-Derived Stem Cells
    Authors: Choong-kun Lee, Bo-Yoon Park, Taehee Jo, Cheol-Heum Park, Ju-Hee Kim, Kyu-Jin Chung et al.
    Stem Cells Translational Medicine
  6. A single-cell atlas of non-haematopoietic cells in human lymph nodes and lymphoma reveals a landscape of stromal remodelling
    Authors: Yoshiaki Abe, Mamiko Sakata-Yanagimoto, Manabu Fujisawa, Hiroaki Miyoshi, Yasuhito Suehara, Keiichiro Hattori et al.
    Nature Cell Biology
  7. Inhibition of the VEGF signaling pathway attenuates tumor?associated macrophage activity in liver cancer
    Authors: S Okikawa, Y Morine, Y Saito, S Yamada, K Tokuda, H Teraoku, K Miyazaki, S Yamashita, T Ikemoto, S Imura, M Shimada
    Oncology reports, 2022-02-16;47(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Migration Assay Control
  8. GluA3 autoantibodies induce alterations in dendritic spine and behavior in mice
    Authors: D Scheggia, J Stanic, M Italia, F La Greca, E Zianni, A Benussi, B Borroni, M Di Luca, F Gardoni
    Brain, Behavior, and Immunity, 2021-07-09;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo Control
  9. Dissecting spatial heterogeneity and the immune-evasion mechanism of CTCs by single-cell RNA-seq in hepatocellular carcinoma
    Authors: YF Sun, L Wu, SP Liu, MM Jiang, B Hu, KQ Zhou, W Guo, Y Xu, Y Zhong, XR Zhou, ZF Zhang, G Liu, S Liu, YH Shi, Y Ji, M Du, NN Li, GB Li, ZK Zhao, XY Huang, LQ Xu, QC Yu, DH Peng, SJ Qiu, HC Sun, M Dean, XD Wang, WY Chung, AR Dennison, J Zhou, Y Hou, J Fan, XR Yang
    Nature Communications, 2021-07-02;12(1):4091.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization Control
  10. Alternol Sensitizes Renal Carcinoma Cells to TRAIL-Induced Apoptosis
    Authors: Yu Ren, Xue Wang, Shuaishuai Huang, Yangkai Xu, Guobin Weng, Rui Yu
    Frontiers in Pharmacology
  11. Synthesis and Biological Evaluation of Novel Isoxazole-Amide Analogues as Anticancer and Antioxidant Agents
    Authors: AM Eid, M Hawash, J Amer, A Jarrar, S Qadri, I Alnimer, A Sharaf, R Zalmoot, O Hammoudie, S Hameedi, A Mousa
    BioMed Research International, 2021-03-09;2021(0):6633297.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. High-Content Analysis-Based Sensitivity Prediction and Novel Therapeutics Screening for c-Met-Addicted Glioblastoma
    Authors: JW Oh, YJ Oh, S Han, NG Her, DH Nam
    Cancers, 2021-01-20;13(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  13. The inhibitory effect of TU-100 on hepatic stellate cell activation in the tumor microenvironment
    Authors: Y Wada, K Tokuda, Y Morine, S Okikawa, S Yamashita, T Ikemoto, S Imura, Y Saito, S Yamada, M Shimada
    Oncotarget, 2020-12-08;11(49):4593-4604.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. An allosteric peptide inhibitor of HIF-1&alpha regulates hypoxia-induced retinal neovascularization
    Authors: A Usui-Ouchi, E Aguilar, S Murinello, M Prins, ML Gantner, PE Wright, RB Berlow, M Friedlande
    Proc Natl Acad Sci U S A, 2020-10-26;0(0):.
    Species: Mouse
    Sample Types: Reference Standard
    Applications: Control
  15. Disruption of the tumour-associated EMP3 enhances erythroid proliferation and causes the MAM-negative phenotype
    Authors: N Thornton, V Karamatic, L Tilley, CA Green, CL Tay, RE Griffiths, BK Singleton, F Spring, P Walser, AG Alattar, B Jones, R Laundy, JR Storry, M Möller, L Wall, R Charlewood, CM Westhoff, C Lomas-Fran, V Yahalom, U Feick, A Seltsam, B Mayer, ML Olsson, DJ Anstee
    Nat Commun, 2020-07-16;11(1):3569.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  16. Nanoparticle interactions with immune cells dominate tumor retention and induce T cell-mediated tumor suppression in models of breast cancer
    Authors: P Korangath, JD Barnett, A Sharma, ET Henderson, J Stewart, SH Yu, SK Kandala, CT Yang, JS Caserto, M Hedayati, TD Armstrong, E Jaffee, C Gruettner, XC Zhou, W Fu, C Hu, S Sukumar, BW Simons, R Ivkov
    Sci Adv, 2020-03-25;6(13):eaay1601.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  17. Adipose Tissue-Derived Mesenchymal Stem Cells Suppress Growth of Huh7 Hepatocellular Carcinoma Cells via Interferon (IFN)-beta -Mediated JAK/STAT1 Pathway in vitro
    Authors: Chun Sung Byun, Soonjae Hwang, Sung-Hun Woo, Moon Young Kim, Jin Suk Lee, Jong In Lee et al.
    International Journal of Medical Sciences
  18. High Throughput Traction Force Microscopy for Multicellular Islands on Combinatorial Microarrays
    Authors: Ian C. Berg, Gregory H. Underhill
    BIO-PROTOCOL
  19. Citrullinated Inhibitor of DNA Binding 1 Is a Novel Autoantigen in Rheumatoid Arthritis
    Authors: Ray A. Ohara, Gautam Edhayan, Stephanie M. Rasmussen, Takeo Isozaki, Henriette A. Remmer, Thomas M. Lanigan et al.
    Arthritis & Rheumatology
  20. Immunoglobulin G responses against falciparum malaria specific antigens are higher in children with homozygous sickle cell trait than those with normal hemoglobin
    Authors: GM Bwire, M Majigo, R Makalla, L Nkinda, A Mawazo, M Mizinduko, J Makani
    BMC Immunol., 2019-04-27;20(1):12.
    Species: Human
    Sample Types: Protein
    Applications: ELISA Standard
  21. ITGA2 as a potential nanotherapeutic target for glioblastoma
    Authors: P Guo, A Moses-Gard, J Huang, ER Smith, MA Moses
    Sci Rep, 2019-04-17;9(1):6195.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  22. Immunoglobulin‑like transcript 4 and human leukocyte antigen‑G interaction promotes the progression of human colorectal cancer
    Authors: Zhaoyang Cai, Lu Wang, Yali Han, Wenwen Gao, Xiaojuan Wei, Rumei Gong et al.
    International Journal of Oncology
  23. C1s Inhibition by BIVV009 (Sutimlimab) Prevents Complement-Enhanced Activation of Autoimmune Human B Cells In Vitro
    Authors: Pavel A. Nikitin, Eileen L. Rose, Tony S. Byun, Graham C. Parry, Sandip Panicker
    The Journal of Immunology
  24. SPINK1 promotes cell growth and metastasis of lung adenocarcinoma and acts as a novel prognostic biomarker
    Authors: Liyun Xu, Changchang Lu, Yanyan Huang, Jihang Zhou, Xincheng Wang, Chaowu Liu et al.
    BMB Reports
  25. Calpain-mediated tau fragmentation is altered in Alzheimer's disease progression
    Authors: HH Chen, P Liu, P Auger, SH Lee, O Adolfsson, L Rey-Bellet, J Lafrance-V, BA Friedman, M Pihlgren, A Muhs, A Pfeifer, J Ernst, G Ayalon, KR Wildsmith, TG Beach, MP van der Br
    Sci Rep, 2018-11-13;8(1):16725.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Immunoprecipitation
  26. ADAM17 inhibition enhances platinum efficiency in ovarian cancer
    Authors: Nina Hedemann, Christoph Rogmans, Susanne Sebens, Daniela Wesch, Manuel Reichert, Dirk Schmidt-Arras et al.
    Oncotarget
  27. Hypoxia-induced activin A diminishes endothelial cell vasculogenic activity
    Authors: S Merfeld-Cl, H Lu, X Wu, KL March, DO Traktuev
    J. Cell. Mol. Med., 2017-08-18;0(0):.
    Species: Human
    Sample Types: Whole Cells
  28. Comprehensive cross-clade characterization of antibody-mediated recognition, complement-mediated lysis and cell-mediated cytotoxicity of HIV-1 envelope specific antibodies towards the eradication of the HIV-1 reservoir
    Authors: S Mujib, J Liu, AKMN Rahman, JA Schwartz, P Bonner, FY Yue, MA Ostrowski
    J. Virol., 2017-07-27;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  29. iPSC-derived mesenchymal stem cells exert SCF-dependent recovery of cigarette smoke-induced apoptosis/proliferation imbalance in airway cells
    Authors: Xiang Li, Yuelin Zhang, Yingmin Liang, Yuting Cui, Sze C. Yeung, Mary S.M. Ip et al.
    Journal of Cellular and Molecular Medicine
  30. HCV RNA Activates APCs via TLR7/TLR8 While Virus Selectively Stimulates Macrophages Without Inducing Antiviral Responses
    Sci Rep, 2016-07-07;6(0):29447.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  31. Combinatorial microenvironmental regulation of liver progenitor differentiation by Notch ligands, TGF beta, and extracellular matrix
    Authors: Kerim B. Kaylan, Viktoriya Ermilova, Ravi Chandra Yada, Gregory H. Underhill
    Scientific Reports
  32. Glycoprotein nonmetastatic melanoma protein B extracellular fragment shows neuroprotective effects and activates the PI3K/Akt and MEK/ERK pathways via the Na+/K+-ATPase.
    Authors: Yoko Ono, Kazuhiro Tsuruma, Masafumi Takata, Masamitsu Shimazawa, Hideaki Hara
    Scientific Reports, 2016-03-18;0(0):2045-2322.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Control
  33. Active immunization with Tocilizumab mimotopes induces specific immune responses.
    Authors: Yang L, Xing R, Li C, Liu Y, Sun L, Liu X, Wang Y
    BMC Biotechnol, 2015-06-02;15(0):46.
    Species: Bacteria - E. Coli
    Sample Types: Virus
    Applications: Isotype Control
  34. Targeting ADAM-17 with an inhibitory monoclonal antibody has antitumour effects in triple-negative breast cancer cells.
    Authors: Caiazza F, McGowan P, Mullooly M, Murray A, Synnott N, O'Donovan N, Flanagan L, Tape C, Murphy G, Crown J, Duffy M
    Br J Cancer, 2015-05-26;112(12):1895-903.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  35. Coronary artery endothelial cells and microparticles increase expression of VCAM-1 in myocardial infarction
    Authors: Christopher E. Radecke, Alexandra E. Warrick, Gagan D. Singh, Jason H. Rogers, Scott I. Armstrong, Ehrin J. Armstrong
    Thrombosis and Haemostasis
  36. Experimental study of endothelial progenitor cells labeled with superparamagnetic iron oxide in vitro
    Authors: MENG-QI WEI, DI-DI WEN, XIAO-YING WANG, YI HUAN, YONG YANG, JIAN XU et al.
    Molecular Medicine Reports
  37. Pericytes contribute to the disruption of the cerebral endothelial barrier via increasing VEGF expression: implications for stroke.
    Authors: Bai Y, Zhu X, Chao J, Zhang Y, Qian C, Li P, Liu D, Han B, Zhao L, Zhang J, Buch S, Teng G, Hu G, Yao H
    PLoS ONE, 2015-04-17;10(4):e0124362.
    Species: Mouse
    Sample Types: Whole Organism
    Applications: Control
  38. Hypoxia down-regulates expression of secretory leukocyte protease inhibitor in bronchial epithelial cells via TGF-beta1.
    Authors: Pahlman L, Jogi A, Gram M, Mori M, Egesten A
    BMC Pulm Med, 2015-03-07;15(0):19.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  39. B7-H1-expressing antigen-presenting cells mediate polarization of protumorigenic Th22 subsets.
    Authors: Kuang D, Xiao X, Zhao Q, Chen M, Li X, Liu R, Wei Y, Ouyang F, Chen D, Wu Y, Lao X, Deng H, Zheng L
    J Clin Invest, 2014-09-17;124(10):4657-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  40. A novel murine cytomegalovirus vaccine vector protects against Mycobacterium tuberculosis.
    Authors: Beverley P, Ruzsics Z, Hey A, Hutchings C, Boos S, Bolinger B, Marchi E, O'Hara G, Klenerman P, Koszinowski U, Tchilian E
    J Immunol, 2014-07-28;193(5):2306-16.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  41. RepSox slows decay of CD34+ acute myeloid leukemia cells and decreases T cell immunoglobulin mucin-3 expression.
    Authors: Jajosky A, Coad J, Vos J, Martin K, Senft J, Wenger S, Gibson L
    Stem Cells Transl Med, 2014-05-22;3(7):836-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  42. Induction and activation of human Th17 by targeting antigens to dendritic cells via dectin-1.
    Authors: Duluc D, Joo H, Ni L, Yin W, Upchurch K, Li D, Xue Y, Klucar P, Zurawski S, Zurawski G, Oh S
    J Immunol, 2014-05-16;192(12):5776-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  43. Pathogenic strains of Acanthamoeba are recognized by TLR4 and initiated inflammatory responses in the cornea.
    Authors: Alizadeh H, Tripathi T, Abdi M, Smith A
    PLoS ONE, 2014-03-14;9(3):e92375.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  44. The aryl hydrocarbon receptor is functionally upregulated early in the course ofvhuman T-cell activation.
    Authors: Prigent L, Robineau M, Jouneau S, Morzadec C, Louarn L, Vernhet L, Fardel O, Sparfel L
    Eur J Immunol, 2014-02-18;44(5):1330-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  45. Generation of neuronal progenitor cells in response to tumors in the human brain.
    Authors: Macas J, Ku M, Nern C, Xu Y, Buhler H, Remke M, Synowitz M, Franz K, Seifert V, Plate K, Kettenmann H, Glass R, Momma S
    Stem Cells, 2014-01-01;32(1):244-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  46. Angiogenesis stimulated by human kallikrein-related peptidase 12 acting via a platelet-derived growth factor B-dependent paracrine pathway.
    Authors: Kryza T, Achard C, Parent C, Marchand-Adam S, Guillon-Munos A, Iochmann S, Korkmaz B, Respaud R, Courty Y, Heuze-Vourc'h N
    FASEB J, 2013-11-13;28(2):740-51.
    Species: Human
    Sample Types: Protein, Whole Cells
    Applications: IHC, Western Blot
  47. Activation of endothelial TLR2 by bacterial lipoprotein upregulates proteins specific for the neutrophil response
    Authors: Kevin Wilhelmsen, Kailin R Mesa, Arun Prakash, Fengyun Xu, Judith Hellman
    Innate Immunity
  48. Anti-Tumour Effects of a Specific Anti-ADAM17 Antibody in an Ovarian Cancer Model In Vivo.
    Authors: Richards FM, Tape CJ, Jodrell DI, Murphy G
    PLoS ONE, 2012-07-11;7(7):e40597.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  49. ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes.
    Authors: Wilhelmsen K, Mesa K, Lucero J, Xu F, Hellman J
    J Biol Chem, 2012-06-15;287(32):26478-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking
  50. Sjogren's syndrome pathological neovascularization is regulated by VEGF-A-stimulated TACE-dependent crosstalk between VEGFR2 and NF-kappaB.
    Authors: Sisto M, Lisi S, Lofrumento DD, D'Amore M, Frassanito MA, Ribatti D
    Genes Immun., 2012-04-19;13(5):411-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  51. Tumor cell marker PVRL4 (nectin 4) is an epithelial cell receptor for measles virus.
    Authors: Noyce RS, Bondre DG, Ha MN
    PLoS Pathog., 2011-08-25;7(8):e1002240.
    Species: Hamster, Human
    Sample Types: Whole Cells
    Applications: Fc Blocking
  52. Inhibition of Cell Proliferation by an Anti-EGFR Aptamer.
    Authors: Li N, Nguyen HH, Byrom M, Ellington AD
    PLoS ONE, 2011-06-08;6(6):e20299.
    Species: Human
    Sample Types: RNA
    Applications: Control
  53. HER2 phosphorylation is maintained by a PKB negative feedback loop in response to anti-HER2 herceptin in breast cancer.
    Authors: Gijsen M, King P, Perera T, Parker PJ, Harris AL, Larijani B, Kong A
    PLoS Biol., 2010-12-21;8(12):e1000563.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  54. Suppression of TNF-alpha and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27.
    Authors: Kalliolias GD, Gordon RA, Ivashkiv LB
    J. Immunol., 2010-10-22;185(11):7047-56.
    Species: Human
    Sample Types: Whole Cells
  55. TSU68 prevents liver metastasis of colon cancer xenografts by modulating the premetastatic niche.
    Authors: Yamamoto M, Kikuchi H, Ohta M, Kawabata T, Hiramatsu Y, Kondo K, Baba M, Kamiya K, Tanaka T, Kitagawa M, Konno H
    Cancer Res., 2008-12-01;68(23):9754-62.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Control
  56. Spatial patterning of liver progenitor cell differentiation mediated by cellular contractility and Notch signaling
    Authors: KB Kaylan, IC Berg, MJ Biehl, A Brougham-C, I Jain, SM Jamil, LH Sargeant, NJ Cornell, LT Raetzman, GH Underhill
    Elife, 2018-12-27;7(0):.

FAQs

  1. What subtype of IgG does Normal Human IgG Control (Catalog # 1-001-A) contain?

    • Normal Human IgG Control (Catalog # 1-001-A) is produced by purification of IgG from Normal human plasma. It may contain more than one subtype of IgG.

View all Isotype Control FAQs

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Normal Human IgG Control
By Anonymous on 02/16/2020
Application: WB Sample Tested: 293T human embryonic kidney cell line Species: Human

IgG was used as negative control.


Normal Human IgG Control
By Alberto Contreras on 02/04/2020
Application: Func Sample Tested: Bladder cancer tissue Species: Human

UM-UC3 bladder cancer cells were transiently transfected with a Notch-reporter Firefly luciferase plasmid, in addition to an internal Renilla control. IgG was used as a negative stimulation control, whereas Jagged 1 was used to induce a Notch-specific response. Increasing concentrations of the Gamma-secretase inhibitor DAPT resulted in a dose-dependent decrease in Notch signal.


Normal Human IgG Control
By Anonymous on 09/22/2019
Application: WB Sample Tested: Recombinant protein Species: Human

Normal Human IgG Control
By Anonymous on 10/27/2018
Application: Flow Sample Tested: MBA-MB-123 human breast cancer cell line Species: Human

Used at 10 ug/mL works perfectly as a human Isotype control for flow cytometry. There is no background binding in live cells that do not express Fc receptor. The attached image shows MFI with and without the isotype control indicating identical signal.