Human EGFR (Research Grade Cetuximab Biosimilar) Antibody

R&D Systems | Catalog # MAB9577

Recombinant Monoclonal Antibody For Research Use Only
R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Neutralization, Bioassay

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # Hu1
View all EGFR Primary Antibodies »

Product Specifications

Immunogen

Human EGF R/ErbB1

Specificity

Detects human EGFR based on Cetuximab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Cetuximab. This product is for research use only.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human EGFR (Research Grade Cetuximab Biosimilar) Antibody

Detection of EGF R/ErbB1 antibody in A431 human epithelial carcinoma cell line antibody by Flow Cytometry.

Detection of EGF R/ErbB1 in A431 human epithelial carcinoma cell line by Flow Cytometry.

A431 human epithelial carcinoma cell line was stained with Human Anti-Human EGF R/ErbB1 (Research Grade Cetuximab Biosimilar) Monoclonal Antibody (Catalog # MAB9577, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Applications for Human EGFR (Research Grade Cetuximab Biosimilar) Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: A431 human epithelial carcinoma cell line

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: EGFR

Cetuximab is a biosimilar directed against the extracellular domain of EGFR. This product constitutes the immunogen used for the anti-Idiotype catalog number MAB9626. The biotinylated antibody (catalog number FAB9577B) can be used in flow cytometry or various assay format to measure EGFR expression and can be detected by conjugated streptavidin.

Long Name

Epidermal Growth Factor Receptor

Alternate Names

EGF R, ErbB, ErbB1, HER-1

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Gene Symbol

EGFR

Additional EGFR Products

Product Documents for Human EGFR (Research Grade Cetuximab Biosimilar) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human EGFR (Research Grade Cetuximab Biosimilar) Antibody

For research use only

Citations for Human EGFR (Research Grade Cetuximab Biosimilar) Antibody

Customer Reviews for Human EGFR (Research Grade Cetuximab Biosimilar) Antibody

There are currently no reviews for this product. Be the first to review Human EGFR (Research Grade Cetuximab Biosimilar) Antibody and earn rewards!

Have you used Human EGFR (Research Grade Cetuximab Biosimilar) Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs for Human EGFR (Research Grade Cetuximab Biosimilar) Antibody

Showing  1 - 4 of 4 FAQs Showing All

  • Q: Does this Biosimilar antibody have the same sequence as its corresponding therapeutic antibody? 

    A: This Biosimilar antibody has the same variable region sequence as the therapeutic antibody, but the constant region is not the same. It contains a proprietary human IgG constant region. 

  • Q: Is the light chain of Human EGFR (Research Grade Cetuximab Biosimilar) Antibody, Catalog # MAB9577,  kappa or lamba?

    A: MAB9577 is a kappa light chain IgG1.

  • Q: What is a Biosimilar antibody?

    A: A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody.  It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody.  For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.

  • Q: What is the light chain for Human EGFR (Research Grade Cetuximab Biosimilar) Antibody, Catalog #s MAB9577 and FAB9577, Clone # Hu1?

    A: Catalog #s MAB9577 and FAB9577 have a kappa light chain.

  • Q: Does this Biosimilar antibody have the same sequence as its corresponding therapeutic antibody? 

    A: This Biosimilar antibody has the same variable region sequence as the therapeutic antibody, but the constant region is not the same. It contains a proprietary human IgG constant region. 

  • Q: Is the light chain of Human EGFR (Research Grade Cetuximab Biosimilar) Antibody, Catalog # MAB9577,  kappa or lamba?

    A: MAB9577 is a kappa light chain IgG1.

  • Q: What is a Biosimilar antibody?

    A: A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody.  It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody.  For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.

  • Q: What is the light chain for Human EGFR (Research Grade Cetuximab Biosimilar) Antibody, Catalog #s MAB9577 and FAB9577, Clone # Hu1?

    A: Catalog #s MAB9577 and FAB9577 have a kappa light chain.

  • Q: Does this Biosimilar antibody have the same sequence as its corresponding therapeutic antibody? 

    A: This Biosimilar antibody has the same variable region sequence as the therapeutic antibody, but the constant region is not the same. It contains a proprietary human IgG constant region. 

  • Q: Is the light chain of Human EGFR (Research Grade Cetuximab Biosimilar) Antibody, Catalog # MAB9577,  kappa or lamba?

    A: MAB9577 is a kappa light chain IgG1.

  • Q: What is a Biosimilar antibody?

    A: A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody.  It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody.  For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.

  • Q: What is the light chain for Human EGFR (Research Grade Cetuximab Biosimilar) Antibody, Catalog #s MAB9577 and FAB9577, Clone # Hu1?

    A: Catalog #s MAB9577 and FAB9577 have a kappa light chain.

  • Q: Does this Biosimilar antibody have the same sequence as its corresponding therapeutic antibody? 

    A: This Biosimilar antibody has the same variable region sequence as the therapeutic antibody, but the constant region is not the same. It contains a proprietary human IgG constant region. 

  • Q: Is the light chain of Human EGFR (Research Grade Cetuximab Biosimilar) Antibody, Catalog # MAB9577,  kappa or lamba?

    A: MAB9577 is a kappa light chain IgG1.

  • Q: What is a Biosimilar antibody?

    A: A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody.  It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody.  For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.

  • Q: What is the light chain for Human EGFR (Research Grade Cetuximab Biosimilar) Antibody, Catalog #s MAB9577 and FAB9577, Clone # Hu1?

    A: Catalog #s MAB9577 and FAB9577 have a kappa light chain.

Showing  1 - 4 of 4 FAQs Showing All
View all FAQs for Antibodies