Anti- Cetuximab (Anti-Idiotype) Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB9626-100
MAB9626-SP
Detection of Cetuximab by Western Blot.
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Anti- Cetuximab (Anti-Idiotype) Antibody Summary

Specificity
Detects Cetuximab in Western blots and direct ELISAs. No cross-reactivity with Adalimumab, Rituximab, Trastuzumab, Brentuximab, Gemtuzumab, Ibritumomab, Tositumomab, Solanezumab, Teprotumumab, and Aducanumab is observed.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2259C
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Full length Cetuximab
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
20 µg/mL
Cetuximab under non-reducing conditions only
ELISA
Detects Cetuximab in direct ELISAs.
 
Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Cetuximab antibody by Western Blot. View Larger

Detection of Cetuximab by Western Blot. Western blot shows Cetuximab, Rituximab, Brentuximab, and Gemtuzumab biosimilars. PVDF membrane was probed with 0.5 µg/mL of Anti- Cetuximab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB9626) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Cetuximab at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of anti-EGFR/ErbB1 (Cetuximab) on A431 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of anti-EGFR/ErbB1 (Cetuximab) on A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with anti-EGFR/ErbB1 (Cetuximab) (Catalog # MAB9577), then anti-Cetuximab (Anti-Idiotype) (Catalog # MAB9626, filled histogram) or Normal Rabbit IgG Control (Catalog # MAB1050, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110).

Specificity of Anti-Cetuximab (Anti-Idiotype) Antibody in Direct ELISA A panel of biosimilars was coated at 1 μg/mL in 96-well polystyrene plates. Anti-Cetuximab (Anti-Idiotype) Antibody (Catalog # MAB9626, 333 ng/mL) detected Cetuximab. No cross-reactivity with Adalimumab, Rituximab, Trastuzumab, Brentuximab, Gemtuzumab, Ibritumomab, Tositumomab, Solanezumab, Teprotumumab, and Aducanumab was observed.

Simple Western View Larger

Detection of Cetuximab by Simple WesternTM. Simple Western lane view shows Cetuximab and Rituximab biosimilars, loaded at 0.2 mg/mL. A specific band was detected for Cetuximab at approximately 174 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Anti- Cetuximab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB9626). This experiment was conducted under non-reducing conditions and using the 66-440 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cetuximab

The anti-Cetuximab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Cetuximab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.

Alternate Names
Cetuximab

Product Datasheets

FAQs

  1. What is an anti-idiotype antibody?

    • An anti-idiotype antibody is specific for the unique variable (antigen binding) region of another antibody.  We have anti-idiotype antibodies to commecially available therapeutic antibodies or  biosimilars.

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