APBA3/Mint-3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-18365

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all APBA3 Primary Antibodies »

Product Specifications

Immunogen

Recognizes region between residues Between Between 1 and 50..

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for APBA3/Mint-3 Antibody - BSA Free

APBA3/Mint-3 Antibody

Immunoprecipitation:[NBP3-18365]-

Immunoprecipitation:[NBP3-18365]-Detection of human APBA3 by western blot of immunoprecipitates. Samples: Whole cell lysate from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-APBA3 antibody used for IP at 6 µg per reaction. APBA3 was also immunoprecipitated by rabbit anti-APBA3 antibody. For blotting immunoprecipitated APBA3, was used at 0. 4 mg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Applications for APBA3/Mint-3 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

8 - 15 ug/mg lysate

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: APBA3

The munc-18 interacting (Mint) protein family is a group of evolutionarily conserved adaptor proteins that function in membrane transport and organization. In mammals, there exist three mint isoforms, Mint 1, 2, and 3. Although there is little amino acid sequence conservation in the amino-terminal half, the carboxy-terminal half of these proteins is highly conserved. Within this conserved portion there exists a phosphotyrosine-binding (PTB) and a PSD-95/DLG-A/ZO-1 (PDZ) domain, which function as protein interaction modules. Mint 1 and 2 appear to be expressed exclusively in the brain and are found to bind to munc-18, an essential component of the synaptic vesicle fusion machinery. Mint 3 is ubiquitously expressed in all tissues and is expressed at the lowest levels in the brain and testis. Studies show that Mint 3 does not interact with munc-18. Mint 3 has been found to interact with the Alzheimer®s Disease-related amyloid precursor protein (APP) and does so through its PTB and PDZ domains. It has been suggested that Mint 3 links APP to other transport machinery components, thereby regulating it transport, endocytosis, and metabolism. Abnormal APP metabolism has been shown to be the cause of an early-onset type of Alzheimer®s disease.

Long Name

Amyloid Beta [A4] Precursor Protein-binding, Family A, Member 3

Alternate Names

MINT3, X11L2

Gene Symbol

APBA3

Additional APBA3 Products

Product Documents for APBA3/Mint-3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for APBA3/Mint-3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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