ApoStat Intracellular Caspase Detection (FITC-VAD-FMK)

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Citations (16)

ApoStat Intracellular Caspase Detection (FITC-VAD-FMK) Summary

Detection of Caspase Activation in Jurkat Cells. Human Jurkat leukemic T cells were cultured in the absence (green) or presence (purple) of 1 µM staurosporine for 3.5 hours and then stained with ApoStat (Catalog # FMK012) for 30 minutes. The cells were harvested, washed, and assayed by flow cytometry to determine the percentage of cells expressing active caspases.

ApoStat is designed to identify and quantify caspase activity in apoptotic cells by flow cytometry. Cells undergoing apotosis are irreversibly labeled with a cell permeable, FITC-conjugated pan-caspase inhibitor (ApoStat). Any unbound reagent diffuses out of the cell and is washed away. Cells are then analyzed by flow cytometry for the presence of bound reagent. Increased fluorescence is an indicator of caspases activity within cells. The detection of active caspases by flow cytometry is a rapid method for identifying cells undergoing apoptosis.

ApoStat Apoptosis Detection Kit

Testing Format: Flow Cytometry
Sample Type: Cultured cells
Size: 100 Tests

Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. They are expressed as latent zymogens and are activated by an autoproteolytic mechanism or by processing by other proteases (frequently other caspases). Human caspases can be subdivided into three functional groups: cytokine activation (caspase-1, -4, -5, and -13), apoptosis initiation (caspase-2, -8, -9, -and -10), and apoptosis execution (caspase-3, -6, and -7).

Caspases are regulated by a variety of stimili, including APAF1, CFLAR/FLIP, NOL3/ARC, and members of the inhibitor of apoptosis (IAP) family such as BIRC1/NAIP, BIRC2/cIAP-1, BIRC3/cIAP-2, BIRC4/XIAP, BIRC5/Survivin, and BIRC7/Livin. IAP activity is modulated by DIABLO/SMAC or PRSS25/HTRA2/Omi. Cell-permeable and irreversible peptide inhibitors are also available for different caspases.


Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Store reagent at ≤ -20 ˚C in the dark upon arrival. Product is stable for 12 months from date of receipt at ≤ -20 ˚C as supplied. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Flow Cytometry

Product Datasheets

Citations for ApoStat Intracellular Caspase Detection (FITC-VAD-FMK)

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Anti-PfGARP activates programmed cell death of parasites and reduces severe malaria
    Authors: DK Raj, A Das Mohapa, A Jnawali, J Zuromski, A Jha, G Cham-Kpu, B Sherman, RM Rudlaff, CE Nixon, N Hilton, AV Oleinikov, O Chesnokov, J Merritt, S Pond-Tor, L Burns, G Jolly, C Ben Mamoun, E Kabyemela, A Muehlenbac, L Lambert, S Orr-Gonzal, NF Gnädig, DA Fidock, S Park, JD Dvorin, N Pardi, D Weissman, BL Mui, YK Tam, JF Friedman, M Fried, PE Duffy, JD Kurtis
    Nature, 2020;0(0):.  2020
  2. Remote Actuation of Apoptosis in Liver Cancer Cells via Magneto-Mechanical Modulation of Iron Oxide Nanoparticles
    Authors: O Lunov, M Uzhytchak, B Smolková, M Lunova, M Jirsa, NM Dempsey, AL Dias, M Bonfim, M Hof, P Jurkiewicz, Y Petrenko, Š Kubinová, A Dejneka
    Cancers (Basel), 2019;11(12):.  2019
  3. Anticancer and Differentiation Properties of the Nitric Oxide Derivative of Lopinavir in Human Glioblastoma Cells
    Authors: MS Basile, E Mazzon, T Krajnovic, D Draca, E Cavalli, Y Al-Abed, P Bramanti, F Nicoletti, S Mijatovic, D Maksimovic
    Molecules, 2018;23(10):.  2018
  4. Differential cytokine withdrawal-induced death sensitivity of effector T cells derived from distinct human CD8(+) memory subsets
    Authors: SE Larsen, K Voss, ED Laing, AL Snow
    Cell Death Discov, 2017;3(0):17031.  2017
  5. Autophagy suppression sensitizes glioma cells to IMP dehydrogenase inhibition-induced apoptotic death
    Authors: Aleksandra Isakovic
    Exp. Cell Res., 2016;0(0):.  2016
  6. Omega-3 fatty acid EPA improves regenerative capacity of mouse skeletal muscle cells exposed to saturated fat and inflammation
    Authors: Amarjit Saini
    Biogerontology, 2016;0(0):.  2016
  7. Synergistic Anticancer Action of Lysosomal Membrane Permeabilization and Glycolysis Inhibition
    J Biol Chem, 2016;0(0):.  2016
  8. In vitro antiglioma action of indomethacin is mediated via AMP-activated protein kinase/mTOR complex 1 signalling pathway
    Authors: Aleksandar Pantovic
    Int. J. Biochem. Cell Biol, 2016;0(0):.  2016
  9. Idarubicin induces mTOR-dependent cytotoxic autophagy in leukemic cells.
    Authors: Ristic B, Bosnjak M, Arsikin K, Mircic A, Suzin-Zivkovic V, Bogdanovic A, Perovic V, Martinovic T, Kravic-Stevovic T, Bumbasirevic V, Trajkovic V, Harhaji-Trajkovic L
    Exp Cell Res, 2014;326(1):90-102.  2014
  10. IL-32gamma delays spontaneous apoptosis of human neutrophils through MCL-1, regulated primarily by the p38 MAPK pathway.
    Authors: Allaeys I, Gymninova I, Canet-Jourdan C, Poubelle P
    PLoS ONE, 2014;9(10):e109256.  2014
  11. Inhibition of mTOR-dependent autophagy sensitizes leukemic cells to cytarabine-induced apoptotic death.
    Authors: Bosnjak M, Ristic B, Arsikin K, Mircic A, Suzin-Zivkovic V, Perovic V, Bogdanovic A, Paunovic V, Markovic I, Bumbasirevic V, Trajkovic V, Harhaji-Trajkovic L
    PLoS ONE, 2014;9(4):e94374.  2014
  12. Combination of arginine deprivation with TRAIL treatment as a targeted-therapy for mesothelioma.
    Authors: Wangpaichitr M, Wu C, Bigford G, Theodoropoulos G, You M, Li Y, Verona-Santos J, Feun L, Nguyen D, Savaraj N
    Anticancer Res, 2014;34(12):6991-9.  2014
  13. Autophagy inhibition uncovers the neurotoxic action of the antipsychotic drug olanzapine.
    Authors: Vucicevic L, Misirkic-Marjanovic M, Paunovic V, Kravic-Stevovic T, Martinovic T, Ciric D, Maric N, Petricevic S, Harhaji-Trajkovic L, Bumbasirevic V, Trajkovic V
    Autophagy, 2014;10(12):2362-78.  2014
  14. Expression and functional heterogeneity of chemokine receptors CXCR4 and CXCR7 in primary patient-derived glioblastoma cells.
    Authors: Liu, Che, Pham, Kien, Luo, Defang, Reynolds, Brent A, Hothi, Parvinde, Foltz, Gregory, Harrison, Jeffrey
    PLoS ONE, 2013;8(3):e59750.  2013
  15. Opposite effects of nanocrystalline fullerene (C(60)) on tumour cell growth in vitro and in vivo and a possible role of immunosupression in the cancer-promoting activity of C(60).
    Authors: Zogovic NS, Nikolic NS, Vranjes-Djuric SD, Harhaji LM, Vucicevic LM, Janjetovic KD, Misirkic MS, Todorovic-Markovic BM, Markovic ZM, Milonjic SK, Trajkovic VS
    Biomaterials, 2009;30(36):6940-6.  2009
  16. MyD88 plays a critical T cell-intrinsic role in supporting CD8 T cell expansion during acute lymphocytic choriomeningitis virus infection.
    Authors: Rahman AH, Cui W, Larosa DF, Taylor DK, Zhang J, Goldstein DR, Wherry EJ, Kaech SM, Turka LA
    J. Immunol., 2008;181(6):3804-10.  2008


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