APP Antibody

Catalog # Availability Size / Price Qty
PPS044
APP Antibody in Data
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APP Antibody Summary

Species Reactivity
Human, Mouse, Rat, Canine, Chicken, Primate
Specificity
This antibody is specific for the ~120 kDa APP in Western blots of rat brain.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
APP
Formulation
100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Amyloid Precursor Protein by Western Blot. View Larger

Detection of Amyloid Precursor Protein by Western Blot. Western blot of rat hippocampal lysate showing specific immunolabeling of the approximately 120 kDa APP protein.

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ ‑20° C is recommended. Product is stable at ≤ ‑20° C for at least 1 year.

Background: APP

APP, or amyloid precursor protein, is a heparin-binding, 120 kDa type I transmembrane glycoprotein that belongs to the APP family of proteins. It is highly conserved across species, showing at least 97% amino acid (aa) identity between human, monkey, rat, mouse and dog. There are almost a dozen potential alternate splice forms for human APP. The neuronal (or standard) form is referred to as APP695. The 695 includes a 17 aa signal peptide and 678 aa mature region. This form contains a multidomain, 610 aa extracellular region (ECD), a 24 aa transmembrane segment (aa 625 - 648), and a short 47 aa cytoplasmic tail. Longer forms of APP, APP751 and APP770 are characterized by the presence of a 57 aa KPI, or Kunitz protease inhibitor domain in the ECD. This has protease-inhibition activity and may be involved in memory. Membrane-bound APP695 undergoes two-stage proteolytic processing. There is an initial cleavage by either an alpha - or beta -secretase, followed by cleavage by a gamma -secretase. The choice of alpha - or beta -secretase may depend upon the APP glycosylation pattern or phosphorylation events. alpha -Secretase cleavage occurs between K612 and L613. This releases a soluble APPa piece that shows proliferative activity with EGF family members on neural progenitor cells and keratinocytes. beta -secretase cleaves between M596 and D597. When followed by gamma -secretase cleavage following position V637, or A639, small 40 or 42 aa A beta peptides are released that may contribute to the formation of amyloid plaques.

References
  1. Suzuki, T. et al. (2006) J. Biochem. 139:949.
  2. Chen, Y. and B.L. Tang (2006) Biochem. Biophys. Res. Commun. 341:1.
  3. Reinhard, C. et al. (2005) EMBO J. 24:3996.
  4. Mendendez-Gonzales, M. et al. (2005) Neurodegenerative Dis. 2:227.
  5. Nakagawa, K. et al. (2006) J. Neurochem. 96:924.
  6. Kang, J. et al. (1987) Nature 325:733.
Long Name
Amyloid Precursor Protein
Entrez Gene IDs
351 (Human); 11820 (Mouse)
Alternate Names
amyloid beta (A4) precursor protein-binding, family B, member 2; amyloid beta A4 precursor protein-binding family B member 2; Amyloid beta precursor protein; Amyloid beta; APP; beta Amyloid; Protease Nexin II

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