APP Antibody

  
  • Species Reactivity
    Human, Mouse, Rat, Canine, Chicken, Primate
  • Specificity
    This antibody is specific for the ~120 kDa APP in Western blots of rat brain.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    APP
  • Formulation
    100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Amyloid Precursor Protein by Western Blot. Western blot of rat hippocampal lysate showing specific immunolabeling of the approximately 120 kDa APP protein.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    For long-term storage, ≤ ‑20° C is recommended. Product is stable at ≤ ‑20° C for at least 1 year.
Background: APP
APP, or amyloid precursor protein, is a heparin-binding, 120 kDa type I transmembrane glycoprotein that belongs to the APP family of proteins. It is highly conserved across species, showing at least 97% amino acid (aa) identity between human, monkey, rat, mouse and dog. There are almost a dozen potential alternate splice forms for human APP. The neuronal (or standard) form is referred to as APP695. The 695 includes a 17 aa signal peptide and 678 aa mature region. This form contains a multidomain, 610 aa extracellular region (ECD), a 24 aa transmembrane segment (aa 625 - 648), and a short 47 aa cytoplasmic tail. Longer forms of APP, APP751 and APP770 are characterized by the presence of a 57 aa KPI, or Kunitz protease inhibitor domain in the ECD. This has protease-inhibition activity and may be involved in memory. Membrane-bound APP695 undergoes two-stage proteolytic processing. There is an initial cleavage by either an alpha - or beta -secretase, followed by cleavage by a gamma -secretase. The choice of alpha - or beta -secretase may depend upon the APP glycosylation pattern or phosphorylation events. alpha -Secretase cleavage occurs between K612 and L613. This releases a soluble APPa piece that shows proliferative activity with EGF family members on neural progenitor cells and keratinocytes. beta -secretase cleaves between M596 and D597. When followed by gamma -secretase cleavage following position V637, or A639, small 40 or 42 aa A beta peptides are released that may contribute to the formation of amyloid plaques.
  • References:
    1. Suzuki, T. et al. (2006) J. Biochem. 139:949.
    2. Chen, Y. and B.L. Tang (2006) Biochem. Biophys. Res. Commun. 341:1.
    3. Reinhard, C. et al. (2005) EMBO J. 24:3996.
    4. Mendendez-Gonzales, M. et al. (2005) Neurodegenerative Dis. 2:227.
    5. Nakagawa, K. et al. (2006) J. Neurochem. 96:924.
    6. Kang, J. et al. (1987) Nature 325:733.
  • Long Name:
    Amyloid Precursor Protein
  • Entrez Gene IDs:
    351 (Human); 11820 (Mouse)
  • Alternate Names:
    amyloid beta (A4) precursor protein-binding, family B, member 2; amyloid beta A4 precursor protein-binding family B member 2; Amyloid beta precursor protein; Amyloid beta; APP; beta Amyloid; Protease Nexin II
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 43  
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Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 23  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Rabbit IgG Antibody

WB AF008 1
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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