AQUApure Phospho-Tetra-Ub Chains (M1, pS65) Protein, CF

R&D Systems | Catalog # UC-750

 
R&D Systems
Discontinued Product
UC-750 has been discontinued. View all Tetra-Ubiquitin products.

Key Product Details

Source

E. coli

Accession Number

P0CG47

Applications

Bioactivity
View all Tetra-Ubiquitin Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Tetra-Ubiquitin protein

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

 

Ub-AQUA analysis:

      p-S65:       93.5%

Predicted Molecular Mass

34 kDa

Activity

Ubiquitin chains vary in length, linkage, and function.  Linear ("Met1" or "M1"), pS65 Tetra-Ubiquitin chains are ideal for investigating Ubiquitin­-binding proteins and as substrates for Ubiquitin­-specific isopeptidases.  Reaction conditions will need to be optimized for each specific application.  IMPORTANT: Heating this product in SDS-­PAGE buffer or terminating reactions containing this product with heated SDS-­PAGE buffer could lead to unexpected, high apparent molecular weight banding or smearing on gels that is not representative of product purity. For optimal results, we recommend incubation in SDS­PAGE buffer + DTT at ≤40 °C for 20 minutes prior to gel electrophoresis

Formulation, Preparation, and Storage

UC-750
Formulation

1 mg/ml (29 μM) in sterile, deionized water
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Tetra-Ubiquitin

Linkage specific phosphorylated Poly-Ubiquitin chains may be used as a substrate for in vitro reactions with deubiquitinating enzymes ("DUB's") that cleave the peptide or isopeptide linkage between adjacent Ubiquitin molecules.  Phosphorylated Poly-Ubiquitin chains can also be used to investigate mechanisms of binding and recognition between the chains and other proteins that contain Ubiquitin-Associated domains (UBAs), Ubiquitin-interacting motifs (UIMs), ZnF's and/or other Ubiquitin-sensing elements.

Linear (Met1-linked) Tetra-Ubiquitin chains phosphorylated at Serine 65 are manufactured using recombinant wild-type human recombinant Ubiquitin and PINK1 kinase. The use of purely enzymatic techniques avoids the potential for contaminating synthetic intermediates.  The purity of each production lot is assessed using the Absolute Quantitation of Ubiquitin method (Ub-AQUA), an LCMS-based technique that provides extremely accurate information on the composition of Poly-Ubiquitin samples.

References

  1. Heo, J.M., Ordureau A., et al. (2015) Mol. Cell   60(1): 7-20
  2. Kirkpatrick D.S., et al. (2006) Nat Cell Biol.  8(7): 700-10
  3. Ordureau, A., et al. (2014) Mol. Cell   56(3): 360–375
  4. Ordureau, A., et al. (2015) Pro. Nat. Acad. of Sci. USA 112(21): 6637–6642
  5. Phu L., et al. (2011) Mol Cell Proteomics 10(5): M110.003756

Alternate Names

TetraUbiquitin, Ub4

Entrez Gene IDs

7314 (Human)

Gene Symbol

UBB

UniProt

P0CG47

Product Documents for AQUApure Phospho-Tetra-Ub Chains (M1, pS65) Protein, CF

Certificate of Analysis

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Product Specific Notices for AQUApure Phospho-Tetra-Ub Chains (M1, pS65) Protein, CF

For research use only

Related Research Areas

NFkB Pathway

Citations for AQUApure Phospho-Tetra-Ub Chains (M1, pS65) Protein, CF

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