ARF1 Antibody (0C5B3)

Name: ARF1 Antibody (0C5B3)
Brand: Novus Biologicals
SKU: NBP3-16782
Price: 389 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16782

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 0C5B3 expressed in HEK293

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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 70-150 of human ARF1 (P84077). GQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRH

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ARF1 Antibody (0C5B3)

Western Blot: ARF1 Antibody (0C5B3) [NBP3-16782]

Western Blot: ARF1 Antibody (0C5B3) [NBP3-16782] - Western blot analysis of extracts of various cell lines, using ARF1 Rabbit mAb (NBP3-16782) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Immunocytochemistry/ Immunofluorescence: ARF1 Antibody (0C5B3) [NBP3-16782] -

Immunocytochemistry/ Immunofluorescence: ARF1 Antibody (0C5B3) [NBP3-16782] - Immunofluorescence analysis of NIH-3T3 cells using ARF1 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for ARF1 Antibody (0C5B3)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ARF1

The ADP-ribosylation factor (Arf) family comprises a group of structurally and functionally conserved 21 kDa proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. Arf is involved in intracellular protein traffic to and within the Golgi complex. Arf has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D. The Arf family is divided functionally into the Arf and the Arf-like (Arl) proteins. Arfs share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily.

Long Name

ADP-ribosylation factor 1

Alternate Names

ADP-ribosylation factor 1

Gene Symbol

ARF1

Additional ARF1 Products

Product Documents for ARF1 Antibody (0C5B3)

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Certificate of Analysis

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Product Specific Notices for ARF1 Antibody (0C5B3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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