Recombinant Human ARF5 His Protein

Novus Biologicals | Catalog # NBP1-72346

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Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE
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Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-180 of Human ARF5

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGLTVSALFS RIFGKKQMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN ICFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV QESADELQKM LQEDELRDAV LLVFANKQDM PNAMPVSELT DKLGLQHLRS RTWYVQATCA TQGTGLYDGL DWLSHELSKR

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

22.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human ARF5 His Protein

SDS-PAGE: Recombinant Human ARF5 His Protein [NBP1-72346]

SDS-PAGE: Recombinant Human ARF5 His Protein [NBP1-72346]

SDS-Page: Recombinant Human ARF5 Protein [NBP1-72346] - 15% SDS-PAGE (3ug)
SDS-PAGE: Recombinant Human ARF5 His Protein [NBP1-72346]

SDS-PAGE: Recombinant Human ARF5 His Protein [NBP1-72346]

SDS-Page: Recombinant Human ARF5 Protein [NBP1-72346] - 15% SDS-PAGE (3ug)

Formulation, Preparation, and Storage

NBP1-72346
Formulation 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 20% glycerol, 0.1 M NaCl
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ARF5

ADP-ribosylation factor 5, also known as ARF5, is a small guanine nucleotide-binding protein that enhances the enzymatic activities of cholera toxin. ARF-dependent regulatory mechanisms include the coordination of spectrin interactions with golgi membranes and the association of actin to the golgi via rho family-dependent G-protein localization and WASP/Arp2/3 complexes. ARF5 is involved in vesicular transport and functioning via phospholipase D activation. Recombinant human ARF5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

ADP-ribosylation factor 5

Gene Symbol

ARF5

Additional ARF5 Products

Product Documents for Recombinant Human ARF5 His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human ARF5 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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FAQs for Recombinant Human ARF5 His Protein

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  • Q: I am looking for antibodies against human ARF4 and ARF5 and I need to be able to distinguish between the two proteins. Do you have antibodies specific for each protein that won't cross react with the other protein?

    A: I checked the sequence similarity between ARF4 and ARF5, and there is very high homology between the two proteins. We have not specifically tested our antibodies for cross-reactivity between the two, but I think it's likely that they will.

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