ARID1A Antibody (HL2304) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-25363
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2304 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant fragment of human ARID1A.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ARID1A Antibody (HL2304) - Azide and BSA Free
Western Blot: ARID1A Antibody (HL2304) - Azide and BSA Free [NBP3-25363] -
Western Blot: ARID1A Antibody (HL2304) - Azide and BSA Free [NBP3-25363] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ARID1A antibody [HL2304] (NBP3-25363) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: ARID1A Antibody (HL2304) - Azide and BSA Free [NBP3-25363] -
Immunohistochemistry-Paraffin: ARID1A Antibody (HL2304) - Azide and BSA Free [NBP3-25363] - ARID1A antibody [HL2304] detects ARID1A protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. ARID1A stained by ARID1A antibody [HL2304] (NBP3-25363) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: ARID1A Antibody (HL2304) - Azide and BSA Free [NBP3-25363] -
Immunohistochemistry-Paraffin: ARID1A Antibody (HL2304) - Azide and BSA Free [NBP3-25363] - ARID1A antibody [HL2304] detects ARID1A protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. ARID1A stained by ARID1A antibody [HL2304] (NBP3-25363) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: ARID1A Antibody (HL2304) - Azide and BSA Free [NBP3-25363] -
Immunohistochemistry-Paraffin: ARID1A Antibody (HL2304) - Azide and BSA Free [NBP3-25363] - ARID1A antibody [HL2304] detects ARID1A protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human hepatocellular carcinoma. ARID1A stained by ARID1A antibody [HL2304] (NBP3-25363) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunocytochemistry/ Immunofluorescence: ARID1A Antibody (HL2304) [NBP3-25363] -
ARID1A antibody [HL2304] detects ARID1A protein by immunofluorescent analysis.Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ARID1A stained by ARID1A antibody [HL2304] (NBP3-25363) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Applications for ARID1A Antibody (HL2304) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ARID1A
Alternate Names
ARID domain-containing protein 1A, AT rich interactive domain 1A (SWI- like), AT rich interactive domain 1A (SWI-like), AT-rich interactive domain-containing protein 1A, B120SWI-like protein, BAF250A, BAF250SWI/SNF complex protein p270, BM029, brain protein 120, BRG1-associated factor 250, BRG1-associated factor 250a, C10rf4, C1orf4SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatinsubfamily F member 1, chromatin remodeling factor p250, hELD, hOSA1, matrix associated, actin dependent regulator of chromatin, Osa homolog 1, OSA1, OSA1 nuclear protein, P270, SMARCF1, subfamily f, member 1
Gene Symbol
ARID1A
Additional ARID1A Products
Product Documents for ARID1A Antibody (HL2304) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ARID1A Antibody (HL2304) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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