Asunaprevir

Tocris Bioscience | Catalog # 7755

Potent hepatitis C virus (HCV) NS3 protease inihibitor
Tocris Bioscience

Key Product Details

Description

Potent hepatitis C virus (HCV) NS3 protease inihibitor
View all CRISPR Reagents »

Product Description

Asunaprevir is a potent HCV NS3 protease inhibitor (IC50 = 0.2-3.5 nM). Asunaprevir can be used to control CRIPSR-Cas9 gene editing via the small molecule-assisted shut-off (SMASh) technique, which involves fusion of Cas9 to a peptide comprising a protease domain and a degron tag. Asuprenavir blocks the protease-mediated removal of the degron tag leading to degradation of Cas9 through proteasome and autophagolysosome pathways. It can also be used as a resversible switch to control CAR (chimeric antigen receptor)-T cells, where the CAR has been modified by insertion of a hepatitis C virus nonstructural protein 3 protease (HCV-NS3) between the scFv and the hinge domains. Also inhibits SARS-CoV-2 Mpro activity (IC50 = 15-48.4 μM).

Product Specifications for Asunaprevir

Molecular Weight

748.29

Formula

C35H46ClN5O9S

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

1-[[N-tert-Butoxycarbonyl-L-tert-leucyl-trans-4-[(7-chloro-4-methoxyisoquinolin-1-yl)oxy]-L-prolyl]amino]-N-(cyclopropylsulfonyl)-(1R,2S)-2-vinylcyclopropane-1-carboxamide

CAS Number

630420-16-5

PubChem ID

16076883

InChI Key

XRWSZZJLZRKHHD-WVWIJVSJSA-N

SMILES

O=C([C@H]1N(C[C@@H](C1)OC2=NC=C(OC)C3=C2C=C(Cl)C=C3)C([C@H](C(C)(C)C)NC(OC(C)(C)C)=O)=O)N[C@]4([C@@H](C4)C=C)C(NS(=O)(C5CC5)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 74.83 100
Ethanol 7.48 10

Preparing Stock Solutions for Asunaprevir

The following data is based on the product molecular weight 748.29.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.34 mL 6.68 mL 13.36 mL
5 mM 0.27 mL 1.34 mL 2.67 mL
10 mM 0.13 mL 0.67 mL 1.34 mL
50 mM 0.03 mL 0.13 mL 0.27 mL

Calculators

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Background References

References are publications that support the biological activity of the product.

Product Documents for Asunaprevir

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Asunaprevir

For research use only

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