ATPase Inhibitory Factor 1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-99177

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all ATPase Inhibitory Factor 1 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human ATPase Inhibitory Factor 1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ATPase Inhibitory Factor 1 Antibody - Azide and BSA Free

Western Blot: ATPase Inhibitory Factor 1 Antibody [NBP2-99177]

Western Blot: ATPase Inhibitory Factor 1 Antibody [NBP2-99177]

Western Blot: ATPase Inhibitory Factor 1 Antibody [NBP2-99177] - Anti-ATPase Inhibitory Factor 1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 12 kDa. Observed band size: 15 kDa (We are unsure as to the identity of these extra bands).
Immunohistochemistry-Paraffin: ATPase Inhibitory Factor 1 Antibody [NBP2-99177]

Immunohistochemistry-Paraffin: ATPase Inhibitory Factor 1 Antibody [NBP2-99177]

Immunohistochemistry-Paraffin: ATPase Inhibitory Factor 1 Antibody [NBP2-99177] - Immunochemical staining of human ATPase Inhibitory Factor 1 in human thyroid cancer with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Immunoprecipitation: ATPase Inhibitory Factor 1 Antibody [NBP2-99177]

Immunoprecipitation: ATPase Inhibitory Factor 1 Antibody [NBP2-99177]

Immunoprecipitation: ATPase Inhibitory Factor 1 Antibody [NBP2-99177] - ATPase Inhibitory Factor 1 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. 4 ul anti-ATPase Inhibitory Factor 1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-ATPase Inhibitory Factor 1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 16 kDa

Applications for ATPase Inhibitory Factor 1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATPase Inhibitory Factor 1

ATPase Inhibitory Factor 1 encodes a mitochondrial ATPase inhibitor. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified.

Alternate Names

ATP synthase inhibitor protein, ATPase inhibitor protein, ATPase inhibitor, mitochondrial, ATPase inhibitory factor 1, ATPIIF(1), ATPIP, IF1, Inhibitor of F(1)F(o)-ATPase, IP, MGC1167, MGC8898

Gene Symbol

ATP5IF1

Additional ATPase Inhibitory Factor 1 Products

Product Documents for ATPase Inhibitory Factor 1 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ATPase Inhibitory Factor 1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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