ATPase Recombinant Protein Antigen
Novus Biologicals | Catalog # NBP1-89709PEP
Key Product Details
Source
Applications
Product Specifications
Description
Source: E. coli
Amino Acid Sequence: ILNHKSKHVEEAVRELISIFEQIYEVKYTGKVGKQSEQRKHVVFGSETEEGENNDYEANIVNEFDTHDKEDEFKKECKEVFAFFSHQLLDSLQKATRLSLD
Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)
This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.
Purity
Predicted Molecular Mass
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Applications
Application Notes
It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.
For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com
Protein / Peptide Type
Formulation, Preparation, and Storage
NBP1-89709PEP
| Formulation | PBS and 1M Urea, pH 7.4. |
| Preservative | No Preservative |
| Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20C. Avoid freeze-thaw cycles. |
Background: ATPase
Alternate Names
Gene Symbol
Additional ATPase Products
Product Documents for ATPase Recombinant Protein Antigen
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ATPase Recombinant Protein Antigen
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
Related Research Areas
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FAQs for ATPase Recombinant Protein Antigen
-
Q: I have phosphate in my enzyme. What can I do?
A: You can dialyse or desalt the enzyme into a phosphate-free buffer. Alternatively, you can use a special resin (PiBind) to remove the phosphate.
-
Q: I have 5% DMSO in my assay. Can I use PiColorLock Gold?
A: Yes, the reagent is designed for drug screening work and other situations that require DMSO.
-
Q: I have a high background in my ATPase assay and I definitely do not have free phosphate in my sample
A: This is almost always due to inadequate mixing of the special stabilizer with the sample and detection reagent. Make sure the stabilizer is pipetted up and down several times to ensure thorough mixing.
-
Q: I would like to measure the conversion of pyrophosphate to phosphate. Can I use the PiColorLock Gold Phosphate Detection System for this purpose?
A: Yes, only the phosphate will give a signal; pyrophosphate will not.
-
Q: I have phosphate in my enzyme. What can I do?
A: You can dialyse or desalt the enzyme into a phosphate-free buffer. Alternatively, you can use a special resin (PiBind) to remove the phosphate.
-
Q: I have 5% DMSO in my assay. Can I use PiColorLock Gold?
A: Yes, the reagent is designed for drug screening work and other situations that require DMSO.
-
Q: I have a high background in my ATPase assay and I definitely do not have free phosphate in my sample
A: This is almost always due to inadequate mixing of the special stabilizer with the sample and detection reagent. Make sure the stabilizer is pipetted up and down several times to ensure thorough mixing.
-
Q: I would like to measure the conversion of pyrophosphate to phosphate. Can I use the PiColorLock Gold Phosphate Detection System for this purpose?
A: Yes, only the phosphate will give a signal; pyrophosphate will not.
-
Q: I have phosphate in my enzyme. What can I do?
A: You can dialyse or desalt the enzyme into a phosphate-free buffer. Alternatively, you can use a special resin (PiBind) to remove the phosphate.
-
Q: I have 5% DMSO in my assay. Can I use PiColorLock Gold?
A: Yes, the reagent is designed for drug screening work and other situations that require DMSO.
-
Q: I have a high background in my ATPase assay and I definitely do not have free phosphate in my sample
A: This is almost always due to inadequate mixing of the special stabilizer with the sample and detection reagent. Make sure the stabilizer is pipetted up and down several times to ensure thorough mixing.
-
Q: I would like to measure the conversion of pyrophosphate to phosphate. Can I use the PiColorLock Gold Phosphate Detection System for this purpose?
A: Yes, only the phosphate will give a signal; pyrophosphate will not.
-
Q: I have phosphate in my enzyme. What can I do?
A: You can dialyse or desalt the enzyme into a phosphate-free buffer. Alternatively, you can use a special resin (PiBind) to remove the phosphate.
-
Q: I have 5% DMSO in my assay. Can I use PiColorLock Gold?
A: Yes, the reagent is designed for drug screening work and other situations that require DMSO.
-
Q: I have a high background in my ATPase assay and I definitely do not have free phosphate in my sample
A: This is almost always due to inadequate mixing of the special stabilizer with the sample and detection reagent. Make sure the stabilizer is pipetted up and down several times to ensure thorough mixing.
-
Q: I would like to measure the conversion of pyrophosphate to phosphate. Can I use the PiColorLock Gold Phosphate Detection System for this purpose?
A: Yes, only the phosphate will give a signal; pyrophosphate will not.