AZ1 USP25/28 inhibitor

Key Product Details

Product Description

AZ1 USP25/28 inhibitor is a selective inhibitor of USP28 (IC₅₀ = 0.6 μM, K_d= 0.2 - 3.7 μM) and USP25 (IC₅₀ = 0.7 μM). AZ1 USP25/28 inhibitor exhibits selectivity for USP25 and USP28 over other DUB family members. The compound dose-dependently reduces c-Myc levels in colon carcinoma cells in vitro and induces cell death (EC₅₀ values are 18 - 20 μM). In a mouse Alzheimer's disease model, AZ1 USP25/28 inhibitor reduces amyloid burden, attenuates microglial activation and improves synaptic and cognitive function.

Product Specifications for AZ1 USP25/28 inhibitor

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for AZ1 USP25/28 inhibitor

The following data is based on the product molecular weight 422.21.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

1 Recalculate

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.37 mL	11.84 mL	23.68 mL
5 mM	0.47 mL	2.37 mL	4.74 mL
10 mM	0.24 mL	1.18 mL	2.37 mL
50 mM	0.05 mL	0.24 mL	0.47 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Zheng Trisomy 21-induced dysregulation of microglial homeostasis in Alzheimer's brains is mediated by USP25. Sci.Adv. 2021 PMID: 33523861
• Zheng USP25 inhibition ameliorates Alzheimer's pathology through the regulation of APP processing and Aβ generation. J.Clin.Invest. 2022 PMID: 35229730
• Wrigley Identification and characterization of dual inhibitors of the USP25/28 deubiquitinating enzyme subfamily. ACS Chem.Biol. 2017 PMID: 29131570

Product Documents for AZ1 USP25/28 inhibitor