BDY 650-X, SE
Chemical Name: (T-4)-[2-[4-[2-[2-[([2,2'-Bi-1H-pyrrol]-5-yl-κN1)methylene]-2H-pyrrol-5-yl-κN]ethenyl]phenoxy]-N-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]acetamidato]difluoroboron
Purity: ≥90%
Biological Activity
BDY 650-X, SE is a fluorescent far-red BDY (BODIPY® or boron-dipyrromethene) dye for the labeling of amines. Displays high fluorescence quantum yield and high extinction coefficient and is relatively insensitive to pH change. This dye contains a seven-atom aminohexanoyl ("X") spacer between the fluorophore and the NHS ester group, reducing the potential for interactions between the fluorophore and conjugated biomolecule. The hydrophobic nature of BDY 650-X makes it ideal for labeling lipids and cell membranes. BDY 650-X also exhibits narrow emission bandwidths and has a relatively long excited-state lifetime, making it ideal for fluorescence polarization assays and two-photon excitation (TPE) microscopy. Excitation maximum ~646 nM; emission maximum ~660 nM.For more information on how BDY 650-X, SE may be used, see our protocol: Conjugation Protocol for Amine Reactive Dyes.
BODIPY® is a registered trademark of Molecular Probes.
Optical Data for BDY 650-X, SE
| Emission Color | Far Red |
|---|---|
| Brightness | |
| λabs | 646 nm |
| λem | 660 nm |
| Extinction Coefficient (ε) | 102000 M-1cm-1 |
| Quantum Yield (φ) | 0.52 |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | No |
| Correction Factor 260 | 0.04 |
| Correction Factor 280 | 0.04 |
| Application | Confocal fluorescence, fluorescence polarization assays and two-photon excitation (TPE) microscopy |
| Alternative For | BDY 630-X, SE; Cy5; Alexa Fluor 647 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
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Additional Information
- This product is predominantly the E isomer with <10% Z isomer present
Background References
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A dendritic single-molecule fluorescent probe that is monovalent, photostable and minimally blinking.
Yang et al.
Nat.Chem., 2013;5:692
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