BDY FL Staurosporine
Tocris Bioscience | Catalog # 7985
Key Product Details
Description
Wavelength
Product Description
BDY FL Staurosporine is a fluorescent kinase-targeting probe. It can be paired with antibodies labelled with TR-FRET donors such as CoraFluor™ 1 (Cat. No. 7920) for high throughput binding and protein quantification assays. BDY FL Staurosporine shows high specificity for tyrosine kinase (TK) and tyrosine kinase-like (TKL) family kinases. Excitation and emission maxima (λ) are 514 and 516 nm, respectively; quantum yield = 0.11; extinction coefficient = 35,000 M-1cm-1.The measured KD values, from TR-FRET based saturation binding assay data, are provided in the accompanying supplementary file (see below).
Supplementary Information
The measured KD values, from TR-FRET based saturation binding assay data, are provided in the accompanying supplementary file.Optical Data
| Emission Color | Green |
| Brightness | |
| λabs | 514 nm |
| λem | 516 nm |
| Extinction Coefficient (ε) | 35400 M-1cm-1 |
| Quantum Yield (φ) | 0.11 |
| Closest Laser line | 488 nm |
| Application | Time-resolved fluorescence energy transfer (TR-FRET) |
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Product Specifications for BDY FL Staurosporine
Molecular Weight
Formula
Storage
Purity
CAS Number
PubChem ID
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 9.02 | 10 |
Preparing Stock Solutions for BDY FL Staurosporine
The following data is based on the product molecular weight 901.82.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 11.09 mL | 55.44 mL | 110.89 mL |
| 0.5 mM | 2.22 mL | 11.09 mL | 22.18 mL |
| 1 mM | 1.11 mL | 5.54 mL | 11.09 mL |
| 5 mM | 0.22 mL | 1.11 mL | 2.22 mL |
Calculators
Background References
References are publications that support the biological activity of the product.
- Hirozane Structure-based rational design of staurosporine-based fluorescent probe with broad-ranging kinase affinity for kinase panel application. Bioorg.Med.Chem.Lett. 2019 PMID: 31526603
Product Documents for BDY FL Staurosporine
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for BDY FL Staurosporine
For research use only
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