BDY TR-X, SE
Tocris Bioscience | Catalog # 5467
Key Product Details
Description
Wavelength
Product Description
BDY TR-X, SE is a fluorescent orange BDY (BODIPY® or boron-dipyrromethene) dye for the labeling of amines. Exhibits similar excitation and emission spectra to dye Texas Red®. Displays high fluorescence quantum yield and high extinction coefficient and is relatively insensitive to pH change. This dye contains a seven-atom aminohexanoyl ("X") spacer between the fluorophore and the NHS ester group, reducing the potential for interactions between the fluorophore and conjugated biomolecule. The hydrophobic nature of BDY TR-X makes it ideal for labeling lipids and cell membranes. BDY TR-X also exhibits narrow emission bandwidths and has a relatively long excited-state lifetime, making it ideal for fluorescence polarization assays and two-photon excitation (TPE) microscopy. Excitation maximum ~588 nM; emission maximum ~616 nM.
For more information on how BDY TR-X, SE may be used, see our protocol: Conjugation Protocol for Amine Reactive Dyes.
BODIPY® is a registered trademark of Molecular Probes.
Optical Data
| Emission Color | Orange |
| λabs | 588 nm |
| λem | 616 nm |
| Extinction Coefficient (ε) | 68000 M-1cm-1 |
| Quantum Yield (φ) | 0.9 |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | No |
| Correction Factor 260 | 0.15 |
| Correction Factor 280 | 0.19 |
| Application | Confocal fluorescence p, fluorescence polarization assays and two-photon excitation (TPE) microscopy |
| Alternative For | 5-TAMRA; Alexa Fluor 555; DyLight 549; TRITC |
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Product Specifications for BDY TR-X, SE
Molecular Weight
Formula
Storage
Purity
Chemical Name
CAS Number
PubChem ID
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 63.45 | 100 |
Preparing Stock Solutions for BDY TR-X, SE
The following data is based on the product molecular weight 634.46.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.58 mL | 7.88 mL | 15.76 mL |
| 5 mM | 0.32 mL | 1.58 mL | 3.15 mL |
| 10 mM | 0.16 mL | 0.79 mL | 1.58 mL |
| 50 mM | 0.03 mL | 0.16 mL | 0.32 mL |
Calculators
Background References
References are publications that support the biological activity of the product.
- Vernall Highly potent and selective fluorescent antagonists of the human adenosine A3 receptor based on the 1,2,4-triazolo[4,3-a]quinoxalin-1-one scaffold. J.Med.Chem. 2012 PMID: 22277057
Product Documents for BDY TR-X, SE
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for BDY TR-X, SE
For research use only
Customer Reviews for BDY TR-X, SE
There are currently no reviews for this product. Be the first to review BDY TR-X, SE and earn rewards!
Have you used BDY TR-X, SE?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review