Recombinant Rat beta-Defensin 3 Protein

Novus Biologicals | Catalog # NBP2-35237

Novus Biologicals
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Key Product Details

Source

E. coli

Applications

Bioactivity, SDS-PAGE
View all beta-Defensin 3 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 41 amino acids corresponding to beta-Defensin 3 Source: E. coli

Uniprot ID: Q32ZI4

Amino Acid Sequence: KKVYNAVSCM TNGGICWLKC SGTFREIGSC GTRQLKCCKK K

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of beta-Defensin 3 as determined by LAL method.

Predicted Molecular Mass

4.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Defensin beta 3 Protein is fully biologically active when compared to standard. Measured by its antimicrobial activity against E. coli. The ED50 for this effect is typically 4-20 ug/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat beta-Defensin 3 Protein

SDS-PAGE: Recombinant Rat beta-Defensin 3 Protein [NBP2-35237]

SDS-PAGE: Recombinant Rat beta-Defensin 3 Protein [NBP2-35237]

SDS-Page: Rat beta-Defensin 3 Protein [NBP2-35237]

Formulation, Preparation, and Storage

NBP2-35237
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: beta-Defensin 3

Defensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses. They are 2-6 kDa proteins and take important roles in innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. Beta-Defensins are expressed on some leukocytes and at epithelial surfaces. They contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Because Beat-defensins are cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca 2+ and Mg2+ ions. Furthermore, they can affect the stability of the membrane. Additionally, they are not only have the ability to strengthen the innate immune system but can also enhance the adaptive immune system by chemotaxis of monocytes, T-lymphocytes, dendritic cells and mast cells to the infection site.

Alternate Names

BD14, BD3, betaDefensin 3, DEFB103A, DEFB103B, DEFB3, HBD-3

Gene Symbol

DEFB103B

Additional beta-Defensin 3 Products

Product Documents for Recombinant Rat beta-Defensin 3 Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Rat beta-Defensin 3 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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