BI 2852 FL

Tocris Bioscience | Catalog # 8110

Pan RAS Tracer
Tocris Bioscience

Key Product Details

Description

Pan RAS Tracer

Wavelength

Excitation = 495 nm, Emission = 520 nm
View all Other Fluorescent Probes »

Product Description

BI 2852 FL is a cell-permeable pan-RAS bioluminescence resonance energy transfer (BRET) fluorescent probe. BI 2852 FL is synthesized by conjugating a fluorophore to a derivative of the reversible SI/II-P KRAS inhibitor, BI 2852. BI 2852 FL is used in competitive BRET format to quantify SII-P engagement to multimeric RAS complexes in live cells.

Optical Data

BI 2852 FL Dye Spectra
λabs 495 nm
λem 520 nm

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Product Specifications for BI 2852 FL

Molecular Weight

1088.04

Formula

C59H64BF2N11O7

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

N-(15-(5,5-Difluoro-7-(1H-pyrrol-2-yl)-5H-5λ4,6λ4-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-3-yl)-13-oxo-3,6,9-trioxa-12-azapentadecyl)-5-(2-((6-((((3-(6-hydroxy-3-oxoisoindolin-1-yl)-1H-indol-2-yl)methyl)amino)methyl)-1H-indol-1-yl)methyl)-1H-imidazol-1-yl)pentanamide

CAS Number

2762758-95-0

PubChem ID

162640683

InChI Key

VINGSJQDAYACET-UHFFFAOYSA-N

SMILES

O=C1NC(C2=CC(O)=CC=C12)C3=C(NC4=C3C=CC=C4)CNCC5=CC6=C(C=C5)C=CN6CC7=NC=CN7CCCCC(NCCOCCOCCOCCNC(CCC8=CC=C9C=C%10C=CC(C%11=CC=CN%11)=[N+]%10[B-](N98)(F)F)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 10.88 10

Preparing Stock Solutions for BI 2852 FL

The following data is based on the product molecular weight 1088.04.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 9.19 mL 45.95 mL 91.91 mL
0.5 mM 1.84 mL 9.19 mL 18.38 mL
1 mM 0.92 mL 4.60 mL 9.19 mL
5 mM 0.18 mL 0.92 mL 1.84 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for BI 2852 FL

Certificate of Analysis

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Product Specific Notices for BI 2852 FL

For research use only

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