BIS-TRIS

Tocris Bioscience | Catalog # 5216

Commonly used Zwitterionic buffer
Tocris Bioscience
Discontinued Product
5216 has been discontinued. View all Buffers, Chelators and Reagents products.

Key Product Details

Description

Commonly used Zwitterionic buffer
View all Buffers, Chelators and Reagents »

Product Description

BIS-TRIS is a commonly used zwitterionic buffer. Working pH range in aqueous solution: 5.8 - 7.2. Used in eletrophoresis protocols.

Product Specifications for BIS-TRIS

Molecular Weight

209.24

Formula

C8H19NO5

Storage

Store at RT

Purity

≥99%

Chemical Name

Bis-(2-hydroxyethyl)aminotris(hydroxymethyl)methane

CAS Number

6976-37-0

PubChem ID

81462

InChI Key

OWMVSZAMULFTJU-UHFFFAOYSA-N

SMILES

OCC(CO)(CO)N(CCO)CCO

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 418.48 2000

Preparing Stock Solutions for BIS-TRIS

The following data is based on the product molecular weight 209.24.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
20 mM 0.24 mL 1.19 mL 2.39 mL
100 mM 0.05 mL 0.24 mL 0.48 mL
200 mM 0.02 mL 0.12 mL 0.24 mL
1000 mM 0.00 mL 0.02 mL 0.05 mL

Calculators

Calculate the mass, volume, or concentration required for a solution.

=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Calculate the dilution required to prepare a stock solution.

x
=
x

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background References

References are publications that support the biological activity of the product.

There are currently no references for this product.

Product Documents for BIS-TRIS

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for BIS-TRIS

For research use only

Customer Reviews for BIS-TRIS (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used BIS-TRIS?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card
Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Media ingredient
    Name: Danlee Enzler
    Verified Customer | Posted 05/01/2018
    Media additive

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Cell Culture Products