Blank Comp-Bead Particles

Novus Biologicals | Catalog # NBP3-00500

Novus Biologicals
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Key Product Details

Applications

Flow Cytometry

Product Summary for Blank Comp-Bead Particles

The Blank Compensation Beads serve as a negative control for setting compensation in multicolor flow cytometry.

Concentration: 10^7 particles / mL

Particle size: 3.0 -3.4 micron

Particle material: polystyrene

Product Specifications

Application Notes

Resuspend by vortexing before use. To achieve optimum particle suspension, sonicate the reagent after vortex mixing.

Scientific Data Images for Blank Comp-Bead Particles

Blank Comp-Bead Particles [NBP3-00500] - Coulter M3 analysis: Mean particle size 3.35 micron.
Blank Comp-Bead Particles [NBP3-00500] - Histograms showing low fluorescence in the Red, Green, and Orange channels.

Formulation, Preparation, and Storage

Formulation

0.016 M PBS (pH 7.4), 0.2% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C in the dark. Do not freeze.

Background: Blank Comp-Bead Particles

Compensation is the mathematical correction for the amount of overlap (spillover) of one fluorochrome's emission spectra into another fluorochrome's channel. To calculate this overlap, record single-color stained controls for each fluorochrome in a multicolor panel. This allows the cytometer to visualize the emission of each fluorochrome in each channel, or detector, and subtract the spillover, generating a matrix to apply to multi-color experimental samples. To ensure accurate calculations, single-color controls need to be as bright, or brighter, than the marker on the cell of interest. With increased use of multi-parametric flow cytometry and the large number of fluorochromes available, it is more important than ever to ensure accurate compensation for reliable interpretation of data and reproducible experimental results.

Compensation beads are a highly desirable alternative to traditional cell-based single-color compensation. Positive beads can come pre-loaded with anti-IgG for conjugated antibody capture of mouse, rat, or hamster origin. Uncoated (negative) beads ensure standardization of the autofluorescence in each channel. Beads are preferable to cell-based compensation for several reasons:

1) No precious sample is wasted, and more sample can be used for experimental acquisition.

2) Since 100% of positive beads are able to capture the antibody, the exact experimental fluorochrome can be used regardless of a marker's cellular expression. Dimly expressed markers may not be bright enough for compensation using cells.

3) Reduced autofluorescence of beads allows for more precise calculation of spectral overlap.

Alternate Names

Comp Beads, Compensation Beads, Flow Cytometry Compensation, Negative Compensation Beads

Additional Blank Comp-Bead Particles Products

Product Documents for Blank Comp-Bead Particles

Certificate of Analysis

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Product Specific Notices for Blank Comp-Bead Particles

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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