Recombinant Human BNP Protein

Novus Biologicals | Catalog # NBP2-34936

Novus Biologicals
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Key Product Details

Source

E. coli

Accession Number

P16860

Applications

SDS-PAGE
View all BNP Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 32 amino acids corresponding to BNP Source: E. coli

Uniprot ID: P16860

Amino Acid Sequence: SPKMVQGSGC FGRKMDRISS SSGLGCKVLR RH

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of BNP as determined by LAL method.

Predicted Molecular Mass

3.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation, and Storage

NBP2-34936
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BNP

Brain Natriuretic Peptide is encoded by the BNP gene located on the Chr.1 in humans. It is firstly discovered in the porcine brain and given this name, but the protein is mainly expressed in the cardiac ventricles in human body after the excessive stretching of cardiomyocytes. The gene expresses a 134 a.a. sequence which contains a 1-26 a.a. signal peptide and 27-134 a.a. Natriuretic peptides B, and the BNP is the 32 a.a. C-terminus of natriuretic peptides B. The BNP can be cleaved in 16 chains and the rHuBNP is 1-32. BNP acts as a cardiac hormone with a variety of functions including natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Additionnaly, it plays a key role in cardiovascular homeostasis, helps restore the body's salt and water balance and improves heart function.

Long Name

Brain Natriuretic Peptide

Alternate Names

BNF, NPPB

Gene Symbol

NPPB

UniProt

P16860

Additional BNP Products

Product Documents for Recombinant Human BNP Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human BNP Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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