Bovine SPAM1 Antibody

  
  • Species Reactivity
    Bovine
  • Specificity
    Detects bovine SPAM1 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 763056
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant bovine SPAM1
    Met1-Thr497
    Accession # AAI10184
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Bovine SPAM1 by Western Blot. Western blot shows Recombinant Bovine SPAM1 (Catalog # 6436-GH) (5 ng/lane). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Bovine SPAM1 Monoclonal Antibody (Catalog # MAB6436) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SPAM1 at approximately 78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SPAM1
Sperm adhesion molecule 1 (SPAM1), also known as PH-20 hyaluronidase (1), is encoded by one of the six hyaluronidase-like genes (1-3). SPAM1 is a GPI‑anchored enzyme located on the sperm surface and inner acrosomal membrane (4). SPAM1 degrades hyaluronic acid (HA), a major structural glycosaminoglycan found in extracellular matrices and basement membranes. The enzyme activity enables sperm to penetrate through the HA-rich cumulus cell layer surrounding the oocyte and therefore facilitates the fertilization process (5-7). Because of the structural similarity between HA (repeating units of GlcA beta 1-3GlcNAc) and chondroitin sulfate (repeating units of GlcA beta 1-3GalNAc), the enzyme is also able to hydrolyze chondroitin sulfate. The bovine SPAM1 is 63% identical to human homologue in sequence.
  • References:
    1. Lathrop, W.F. et al. (1990) J. Cell Biol. 111:2939.
    2. Lin, Y. et al. (1993) Proc. Natl. Acad. Sci. U.S.A. 90:10071
    3. Csoka, A.B. et al. (2001) Matrix Biol. 20:499.
    4. Phelps, B.M. et al (1988) Science 240:4860
    5. Gmachl, M. et al. (1993) FEBS Lett. 336:545.
    6. Hofinger, E.S et al. (2007) Glycobiology 17:963.
    7. Wu, Z.L. et al. (2010) BMC Biotechnol. 10:11. 8.
  • Long Name:
    Sperm Adhesion Molecule 1
  • Entrez Gene IDs:
    6677 (Human); 353352 (Bovine)
  • Alternate Names:
    EC 3.2.1.35; HYA1; HYAL1; HYAL3; HYAL5; hyal-PH20; hyaluronidase PH-20; Hyaluronoglucosaminidase PH-20; MGC26532; PH20; PH-20; SPAG15; SPAM1; sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding); Sperm adhesion molecule 1; Sperm surface protein PH-20
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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