Human Breast Tissue Lysate (Adult Tumor)
Novus Biologicals | Catalog # NBP2-28016
Key Product Details
Species
Product Summary for Human Breast Tissue Lysate (Adult Tumor)
Product Specifications
Application Notes
Note: For use in 1D and 2D gel electrophoresis, the addition of a denaturing gel loading buffer with reducing agents may be required.
Specificity
Age :60
Grade:3
Stage:II
TNM:T2N1M0
Clinical Tissue
Human Breast Tumor Tissue Lysate, Soluble protein fraction
Type
Life Stage
Tissue
Tissue Condition
Scientific Data Images for Human Breast Tissue Lysate (Adult Tumor)
Formulation, Preparation, and Storage
Preparation Method
Concentration
Shipping
Storage
Product Documents for Human Breast Tissue Lysate (Adult Tumor)
Certificate of Analysis
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Product Specific Notices for Human Breast Tissue Lysate (Adult Tumor)
The vial is provided with a 10% overfill. Maximum recovery can be obtained by centrifuging the vial briefly to collect any solution on the cap and tube sides.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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Protocols
View specific protocols for Human Breast Tissue Lysate (Adult Tumor) (NBP2-28016):
Lysate Preparation: Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and
centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less
soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to
insolubility of buffer components. The solution should clear upon warming to room temperature.
Extraction 1: PBS, pH 7.4 1 ug/ml Aprotinin 1 mM NaF
Modified RIPA Buffer: 1 mM EDTA 1 ug/ml Pepstatin-A 0.1% SDS
0.25% Na deoxycholate 1 ug/ml Leupeptin 1 mM PMSF
1 mM Na3VO4
Extraction 2: PBS, pH 7.4, 5.0 M Urea, 2.0 M Thiourea, 50mM DTT, 0.1% SDS