Bromodeoxyuridine/BrdU Antibody (BRD.3) - IHC-Prediluted

Novus Biologicals | Catalog # NBP2-48422

Novus Biologicals
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Key Product Details

Species Reactivity

Non-species specific

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # BRD.3

Format

IHC-Prediluted
View all Bromodeoxyuridine/BrdU Primary Antibodies »

Product Specifications

Immunogen

Bromodeoxyuridine/BrdU conjugated to BSA

Localization

Nuclear

Marker

Proliferation Marker

Specificity

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for Bromodeoxyuridine/BrdU Antibody (BRD.3) - IHC-Prediluted

Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (BRD.3) - IHC-Prediluted [NBP2-48422]

Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (BRD.3) - IHC-Prediluted [NBP2-48422]

Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (BRD.3) - IHC-Prediluted [NBP2-48422] - Mouse Small Intestine stained with BrdU Monoclonal Antibody (BRD.3).

Applications for Bromodeoxyuridine/BrdU Antibody (BRD.3) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37C.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Bromodeoxyuridine/BrdU

Bromodeoxyuridine (BrdU) is a synthetic nucleoside analogue of thymidine which can be incorporated into DNA in proliferating cells. During mitosis, BrdU may be incorporated into the newly synthezised DNA of S-phase cells, and can therefore provide an estimate for the fraction of cells in S-phase. Labeling cells with BrdU and then screening with BrdU antibodies has become a powerful tool to study the cytokinetics of normal and neoplastic cells. In vivo, BrdU is considered a potential carcinogen, as its incorporation into DNA may cause mutations.

Alternate Names

5-bromo-2-deoxyuridine, BrdU

Additional Bromodeoxyuridine/BrdU Products

Product Documents for Bromodeoxyuridine/BrdU Antibody (BRD.3) - IHC-Prediluted

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Bromodeoxyuridine/BrdU Antibody (BRD.3) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Bromodeoxyuridine/BrdU Antibody (BRD.3) - IHC-Prediluted

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  • Q: I have been using your BrdU antibody for some time now and saw that you have discontinued it. I was wondering if you had any alternative antibodies that would also work? it is antibody NB500-169 lot# 1706-5

    A:

    We recommend this product: NB500-235

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