Bromodeoxyuridine/BrdU Antibody (BRD.3) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-92989

Novus Biologicals
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Key Product Details

Species Reactivity

Non-species specific

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Immunofluorescence, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # BRD.3

Format

Azide and BSA Free
View all Bromodeoxyuridine/BrdU Primary Antibodies »

Product Specifications

Immunogen

Bromodeoxyuridine/BrdU conjugated to BSA

Localization

Nuclear

Marker

Proliferation Marker

Specificity

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-45221).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Bromodeoxyuridine/BrdU Antibody (BRD.3) - Azide and BSA Free

Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (BRD.3) - Azide and BSA Free [NBP2-92989]

Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (BRD.3) - Azide and BSA Free [NBP2-92989]

Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (BRD.3) - Azide and BSA Free [NBP2-92989] - Formalin-fixed, paraffin-embedded Mouse Small Intestine stained with Bromodeoxyuridine/BrdU Mouse Monoclonal Antibody (BRD.3).

Applications for Bromodeoxyuridine/BrdU Antibody (BRD.3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

1-2ug/ml

Immunohistochemistry-Paraffin

1-2ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37C.
Optimal dilution for a specific application should be determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Bromodeoxyuridine/BrdU

Bromodeoxyuridine (BrdU) is a synthetic nucleoside analogue of thymidine which can be incorporated into DNA in proliferating cells. During mitosis, BrdU may be incorporated into the newly synthezised DNA of S-phase cells, and can therefore provide an estimate for the fraction of cells in S-phase. Labeling cells with BrdU and then screening with BrdU antibodies has become a powerful tool to study the cytokinetics of normal and neoplastic cells. In vivo, BrdU is considered a potential carcinogen, as its incorporation into DNA may cause mutations.

Alternate Names

5-bromo-2-deoxyuridine, BrdU

Additional Bromodeoxyuridine/BrdU Products

Product Documents for Bromodeoxyuridine/BrdU Antibody (BRD.3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Bromodeoxyuridine/BrdU Antibody (BRD.3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle

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Protocols

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FAQs for Bromodeoxyuridine/BrdU Antibody (BRD.3) - Azide and BSA Free

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  • Q: I have been using your BrdU antibody for some time now and saw that you have discontinued it. I was wondering if you had any alternative antibodies that would also work? it is antibody NB500-169 lot# 1706-5

    A:

    We recommend this product: NB500-235

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