BubR1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-18818

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Format

BSA Free
View all BubR1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein corresponding to amino acids 232-603 of the mouse Bub1b protein.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for BubR1 Antibody - BSA Free

Western Blot: BubR1 Antibody [NBP3-18818]

Western Blot: BubR1 Antibody [NBP3-18818]

Western Blot: BubR1 Antibody [NBP3-18818] - Immunoprecipitation/ Western blot analysis on NIH/3T3 cells synchronized to obtain mostly mitotic cells as determined by flow cytometry

Applications for BubR1 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1-10 ug/ml
Application Notes
Detects Bub1b in mitotic NIH/3T3 cells, seperated by immunoprecipitation. Westen blot analysis (1-10 ug/ml) detects a 130 kDa band. Optimal antibody concentration should be evaluated by serial dilutions..

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

BSA Free

Preservative

0.08% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BubR1

Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (BUB1B), also known as BubR1, is a kinase involved in spindle checkpoint function. BUBR1 is essential for normal mitotic progression as it prevents cells from entering into anaphase too early. BUBR1 has been detected in human cancers. BubR-1 localizes to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation.

BubR1 antibodies are useful tools for cancer research and cell division studies.

Alternate Names

Bub1A, BUBR1budding uninhibited by benzimidazoles 1 (yeast homolog), beta, budding uninhibited by benzimidazoles 1 homolog beta (yeast), EC 2.7.11.1, hBUBR1, MAD3/BUB1-related protein kinase, MAD3LBUB1beta, Mitotic checkpoint kinase MAD3L, Protein SSK1, SSK1mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Gene Symbol

BUB1B

UniProt

Q9Z1S0 (Mouse)

Additional BubR1 Products

Product Documents for BubR1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for BubR1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for BubR1 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review BubR1 Antibody - BSA Free and earn rewards!

Have you used BubR1 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies