C5L2/GPR77 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86777

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all C5L2/GPR77 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NDSVSYEYGDYSDLSDRPVDCLDGACLAIDPLR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for C5L2/GPR77 Antibody - BSA Free

Immunohistochemistry-Paraffin: C5L2/GPR77 Antibody [NBP1-86777]

Immunohistochemistry-Paraffin: C5L2/GPR77 Antibody [NBP1-86777]

Immunohistochemistry-Paraffin: C5L2/GPR77 Antibody [NBP1-86777] - Staining of human small intestine shows distinct positivity in subset of glandular cells.
C5L2/GPR77 Antibody - BSA Free Western Blot: C5L2/GPR77 Antibody - BSA Free [NBP1-86777]

Western Blot: C5L2/GPR77 Antibody - BSA Free [NBP1-86777]

Analysis in control (vector only transfected HEK293T lysate) and C5AR2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Applications for C5L2/GPR77 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: C5L2/GPR77

Official Gene Symbol: GPR77 Gen Bank Accession Number: NP_060955 Gene ID: 27202 (human) Gene Map Locus: 19q13.33 (human) C5L2 is 337A.A orphan member that belongs to anaphylatoxin receptor family. It is a seven transmembrane G-protein-coupled receptor consisting of an N-terminal N-glycosylation site, several conserved cysteines and several potential C-terminal phosphorylation sites. It binds to C5a and C5a-des-Arg and serves as a highly regulated scavenger receptor with a negative modulatory influence on C5aR-mediated inflammation. Northern Blot analysis suggests it is co- expressed along with C5aR in different tissues and cells including neutrophils/macrophages, mast cells, dendritic cells, brain, lung, heart, kidney, liver, ovary and testis.

Long Name

C5a Anaphylatoxin Chemotactic Receptor C5L2

Alternate Names

GPR77

Gene Symbol

C5AR2

Additional C5L2/GPR77 Products

Product Documents for C5L2/GPR77 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for C5L2/GPR77 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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