Human CA125/MUC16 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-59961

Novus Biologicals
Discontinued Product
NBP2-59961 has been discontinued. View all CA125/MUC16 products.

Key Product Details

Sample Type & Volume Required Per Well

Serum and Plasma (50 - 100 uL)
View all CA125/MUC16 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This assay has high sensitivity and excellent specificity for detection of human CA125. No significant cross-reactivity or interference between human CA125 and analogues was observed.

Description

This ELISA kit has been transitioned to a new version, available here: DCA125

Precision

Intra-Assay Precision (Precision within an assay) %CV 15

Inter-Assay Precision (Precision between assays) %CV 20

Scientific Data Images for Human CA125/MUC16 ELISA Kit (Colorimetric)

Human CA125/MUC16 ELISA Kit (Colorimetric)

Human CA125/MUC16 ELISA Kit (Colorimetric) [NBP2-59961] -

Human CA125/MUC16 ELISA Kit (Colorimetric) [NBP2-59961] - Standard Curve Reference

Kit Contents for Human CA125/MUC16 ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate
  • HRP-conjugate
  • Instruction manual
  • Standard
  • Stop Solution
  • Substrate A
  • Substrate B
  • Wash Buffer (20 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CA125/MUC16

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CA125 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for CA125. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CA125 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Long Name

Ovarian Cancer Antigen CA125

Alternate Names

MUC16

Gene Symbol

MUC16

UniProt

Q8WXI7

Additional CA125/MUC16 Products

Product Documents for Human CA125/MUC16 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human CA125/MUC16 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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