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Human CA125/MUC16 Quantikine ELISA Kit

R&D Systems | Catalog # DCA125

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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Saliva (10 µL), Urine (100 µL), Human Milk (10 µL)

Sensitivity

0.099 U/mL

Assay Range

0.5-32 U/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
View all CA125/MUC16 ELISA Kits »

Product Summary for Human CA125/MUC16 Quantikine ELISA Kit

The Quantikine Human CA125/MUC16 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CA125 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains CHO cell-expressed recombinant human CA125 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CA125 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CA125.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human CA125

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Human Milk, Saliva, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 4.26 10.0 19.0 4.22 9.74 18.7
Standard Deviation 0.066 0.101 0.266 0.313 0.527 0.666
CV% 1.5 1.0 1.4 7.4 5.4 3.6

Recovery for Human CA125/MUC16 Quantikine ELISA Kit

The recovery of CA125 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 99 95-102
EDTA Plasma (n=4) 97 93-101
Heparin Plasma (n=4) 97 92-100
Serum (n=4) 97 90-101

Linearity

To assess the linearity of the assay, samples containing high concentrations of CA125 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human CA125/MUC16 ELISA Linearity

Scientific Data Images for Human CA125/MUC16 Quantikine ELISA Kit

Human CA125/MUC16 ELISA Standard Curve

Human CA125/MUC16 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CA125/MUC16

The CA125 antigen, also known as MUC16, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. Expression of isoforms, proteolytic cleavage, and heavy N- and O- linked glycosylation produce forms of human CA125 that can vary from 200-5000 kDa in size. It inhibits NK cell activity, which is thought to contribute to immune evasion in peritoneal cancer and pregnancy.

Long Name

Ovarian Cancer Antigen CA125

Alternate Names

MUC16

Entrez Gene IDs

94025 (Human)

Gene Symbol

MUC16

Additional CA125/MUC16 Products

Product Documents for Human CA125/MUC16 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CA125/MUC16 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human CA125/MUC16 Quantikine ELISA Kit

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  • Human CA125/MUC16 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 05/24/2024
  • Human CA125/MUC16 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 12/02/2023
    Human serum
    Human CA125/MUC16 Quantikine ELISA Kit DCA125
  • Human CA125/MUC16 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 12/01/2023
    Human CA125/MUC16 Quantikine ELISA Kit DCA125
  • Human CA125/MUC16 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 10/04/2020
  • Human CA125/MUC16 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: peritoneal fluid
    Verified Customer | Posted 05/26/2020
    Human CA125/MUC16 Quantikine ELISA Kit DCA125

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Protocols

View specific protocols for Human CA125/MUC16 Quantikine ELISA Kit (DCA125):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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