Human CA125/MUC16 Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
Human CA125/MUC16 ELISA Standard Curve
2 Images
Product Details
Citations (7)
Supplemental Products
Reviews (2)

Human CA125/MUC16 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (10 uL), Urine (100 uL), Human Milk (10 uL)
0.099 U/mL
Assay Range
0.5 - 32 U/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Natural and recombinant human CA125
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human CA125/MUC16 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CA125 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains CHO cell-expressed recombinant human CA125 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CA125 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CA125.


Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 4.26 10 19 4.22 9.74 18.7
Standard Deviation 0.066 0.101 0.266 0.313 0.527 0.666
CV% 1.5 1 1.4 7.4 5.4 3.6


The recovery of CA125 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 99 95-102
EDTA Plasma (n=4) 97 93-101
Heparin Plasma (n=4) 97 92-100
Serum (n=4) 97 90-101


To assess the linearity of the assay, samples containing high concentrations of CA125 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human CA125/MUC16 ELISA Linearity

Scientific Data

Human CA125/MUC16 ELISA Standard Curve

Product Datasheets

You must select a language.


Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CA125/MUC16

The CA125 antigen, also known as MUC16, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. Expression of isoforms, proteolytic cleavage, and heavy N- and O- linked glycosylation produce forms of human CA125 that can vary from 200-5000 kDa in size. It inhibits NK cell activity, which is thought to contribute to immune evasion in peritoneal cancer and pregnancy.

Long Name:
Ovarian Cancer Antigen CA125
Entrez Gene IDs:
94025 (Human)
Alternate Names:
CA125 ovarian cancer antigen; CA125; CA-125; CA125MUC-16; FLJ14303; MUC16; mucin 16, cell surface associated; mucin-16; Ovarian cancer-related tumor marker CA125; Ovarian carcinoma antigen CA125
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human CA125/MUC16 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
Filter your results:

Filter by:

  1. Plasma circN4BP2L2 is a promising novel diagnostic biomarker for epithelial ovarian cancer
    Authors: L Ning, J Lang, L Wu
    BMC Cancer, 2022;22(1):6.
    Species: Human
    Sample Types: Serum
  2. A novel circulating miRNA panel for non-invasive ovarian cancer diagnosis and prognosis
    Authors: AW Gahlawat, T Witte, L Haarhuis, S Schott
    British Journal of Cancer, 2022;0(0):.
    Species: Human
    Sample Types: Plasma
  3. Diagnostic value of circN4BP2L2 in type I and type II epithelial ovarian cancer
    Authors: L Ning, J Lang, B Long, L Wu
    BMC Cancer, 2022;22(1):1210.
    Species: Human
    Sample Types: Plasma
  4. Tumor characterization by ultrasound-release of multiple protein and microRNA biomarkers, preclinical and clinical evidence
    Authors: AL D'Souza, JR Chevillet, P Ghanouni, X Yan, M Tewari, SS Gambhir
    PLoS ONE, 2018;13(3):e0194268.
    Species: Human
    Sample Types: Plasma
  5. Novel three dimensional human endocervix cultures respond to 28-day hormone treatment.
    Authors: Arslan S, Yu Y, Burdette J, Pavone M, Hope T, Woodruff T, Kim J
    Endocrinology, 2015;156(4):1602-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. False biomarker discovery due to reactivity of a commercial ELISA for CUZD1 with cancer antigen CA125.
    Authors: Prassas, Ioannis, Brinc, Davor, Farkona, Sofia, Leung, Felix, Dimitromanolakis, Apostolo, Chrystoja, Caitlin, Brand, Randall, Kulasingam, Vathany, Blasutig, Ivan M, Diamandis, Elefther
    Clin Chem, 2014;60(2):381-8.
    Species: Human
    Sample Types: Serum
    Applications: Western Blot
  7. Kallikrein family proteases KLK6 and KLK7 are potential early detection and diagnostic biomarkers for serous and papillary serous ovarian cancer subtypes.
    Authors: Tamir, Ayala, Jag, Ushma, Sarojini, Sreeja, Schindewolf, Craig, Tanaka, Takemi, Gharbaran, Rajendra, Patel, Hiren, Sood, Anil, Hu, Wei, Patwa, Ruzeen, Blake, Patrick, Chirina, Polina, Oh Jeong, Jin, Lim, Heejin, Goy, Andre, Pecora, Andrew, Suh, K Stephe
    J Ovarian Res, 2014;7(1):109.
    Species: Human
    Sample Types: Serum


No product specific FAQs exist for this product, however you may

View all ELISA FAQs

Supplemental ELISA Products

Reviews for Human CA125/MUC16 Quantikine ELISA Kit

Average Rating: 5 (Based on 2 Reviews)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Human CA125/MUC16 Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Human CA125/MUC16 Quantikine ELISA Kit
By Anonymous on 10/04/2020
Sample Tested: Serum

Human CA125/MUC16 Quantikine ELISA Kit
By Anonymous on 05/26/2020
Sample Tested: peritoneal fluid