Calpain 1 Antibody (10E6O5)

Novus Biologicals | Catalog # NBP3-16687

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10E6O5 expressed in HEK293
View all Calpain 1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Calpain 1 (P07384). DGATRTDICQGALGDCWLLAAIASLTLNDTLLHRVVPHGQSFQNGYAGIFHFQLWQFGEWVDVVVDDLLPIKDGKLVFVHSAEGNEFWSALLEKAYAKVNG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Calpain 1 Antibody (10E6O5)

Western Blot: Calpain 1 Antibody (10E6O5) [NBP3-16687]

Western Blot: Calpain 1 Antibody (10E6O5) [NBP3-16687]

Western Blot: Calpain 1 Antibody (10E6O5) [NBP3-16687] - Analysis of extracts of various cell lines, using Calpain 1 Rabbit mAb (NBP3-16687) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Calpain 1 Antibody (10E6O5)

Immunocytochemistry/ Immunofluorescence: Calpain 1 Antibody (10E6O5) [NBP3-16687] -

Immunocytochemistry/ Immunofluorescence: Calpain 1 Antibody (10E6O5) [NBP3-16687] - Immunofluorescence analysis of NIH/3T3 cells using Calpain 1 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Calpain 1 Antibody (10E6O5)

Immunocytochemistry/ Immunofluorescence: Calpain 1 Antibody (10E6O5) [NBP3-16687] -

Immunocytochemistry/ Immunofluorescence: Calpain 1 Antibody (10E6O5) [NBP3-16687] - Immunofluorescence analysis of C6 cells using Calpain 1 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Calpain 1 Antibody (10E6O5)

Western Blot: Calpain 1 Antibody (10E6O5) [NBP3-16687] -

Western Blot: Calpain 1 Antibody (10E6O5) [NBP3-16687] - Western blot analysis of extracts of various cell lines, using Calpain 1 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3min.

Applications for Calpain 1 Antibody (10E6O5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Calpain 1

The calpain (calcium-dependent protease or calcium-activated neutral protease) system consists of two ubiquitous forms of calpain (Mu Calpain and M Calpain), a tissue specific calpain (N Calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.Mu and M Calpains are heterodimers consisting of 28 kDa and 80 kDa subunits. The 28 kDa subunit is identical in the two isoforms, but the 80 kDa subunits differ with ~50% sequence similarity. 28 kDa / 80 kDa complexes are thought to be inactive proenzymes which, upon binding of calcium, undergo conformational changes that promotes cleavage of the 28 kDa subunit and results in enzyme activation.

Long Name

Calpain-1 catalytic subunit

Alternate Names

Calpain mu-type, CANP 1, CANPL1, CAPN1, EC 3.4.22.52, Micromolar-calpain, muCANP, PIG30

Gene Symbol

CAPN1

Additional Calpain 1 Products

Product Documents for Calpain 1 Antibody (10E6O5)

Certificate of Analysis

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Product Specific Notices for Calpain 1 Antibody (10E6O5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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