Calpain 9 Antibody

Novus Biologicals | Catalog # NBP2-14896

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Calpain 9. The exact sequence is proprietary.

Reactivity Notes

Mouse (89%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Calpain 9 Antibody

Western Blot: Calpain 9 Antibody [NBP2-14896]

Western Blot: Calpain 9 Antibody [NBP2-14896]

Western Blot: Calpain 9 Antibody [NBP2-14896] - Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE, antibody diluted at 1:3000.
Calpain 9 Antibody

Immunocytochemistry/ Immunofluorescence: Calpain 9 Antibody [NBP2-14896] -

Calpain 9 antibody [N1N3] detects Calpain 9 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calpain 9 protein stained by Calpain 9 antibody [N1N3] (NBP2-14896) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for Calpain 9 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Calpain 9

Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The protein encoded by this gene is expressed predominantly in stomach and small intestine and may have specialized functions in the digestive tract. This gene is thought to be associated with gastric cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

calpain 9, calpain 9 (nCL-4), calpain-9, Digestive tract-specific calpain, EC 3.4.22, EC 3.4.22.54, GC36, NCL4, nCL-4EC 3.4.22.-, New calpain 4, novel calpain large subunit-4, Protein CG36

Gene Symbol

CAPN9

UniProt

Additional Calpain 9 Products

Product Documents for Calpain 9 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Calpain 9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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