Calpain 9 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-48545

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: PGEYILIPSTFEPHQEADFCLRIFSEKKAITRDMDGNVDIDLPEPPKPTPPDQETEEEQRFRALFEQVAGEDMEVTAEELEYVLNAVLQKKKDIKFKKLS

Reactivity Notes

Mouse (82%), Rat (80%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Calpain 9 Antibody - BSA Free

Immunohistochemistry-Paraffin: Calpain 9 Antibody [NBP2-48545]

Immunohistochemistry-Paraffin: Calpain 9 Antibody [NBP2-48545]

Immunohistochemistry-Paraffin: Calpain 9 Antibody [NBP2-48545] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: Calpain 9 Antibody [NBP2-48545]

Immunohistochemistry-Paraffin: Calpain 9 Antibody [NBP2-48545]

Immunohistochemistry-Paraffin: Calpain 9 Antibody [NBP2-48545] - Analysis in human stomach and pancreas tissues. Corresponding CAPN9 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Calpain 9 Antibody [NBP2-48545]

Immunohistochemistry-Paraffin: Calpain 9 Antibody [NBP2-48545]

Immunohistochemistry-Paraffin: Calpain 9 Antibody [NBP2-48545] - Staining of human fallopian tube shows weak cytoplasmic positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: Calpain 9 Antibody [NBP2-48545]

Immunohistochemistry-Paraffin: Calpain 9 Antibody [NBP2-48545]

Immunohistochemistry-Paraffin: Calpain 9 Antibody [NBP2-48545] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Calpain 9 Antibody [NBP2-48545]

Immunohistochemistry-Paraffin: Calpain 9 Antibody [NBP2-48545]

Immunohistochemistry-Paraffin: Calpain 9 Antibody [NBP2-48545] - Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.

Applications for Calpain 9 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calpain 9

Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The protein encoded by this gene is expressed predominantly in stomach and small intestine and may have specialized functions in the digestive tract. This gene is thought to be associated with gastric cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Alternate Names

calpain 9, calpain 9 (nCL-4), calpain-9, Digestive tract-specific calpain, EC 3.4.22, EC 3.4.22.54, GC36, NCL4, nCL-4EC 3.4.22.-, New calpain 4, novel calpain large subunit-4, Protein CG36

Gene Symbol

CAPN9

Additional Calpain 9 Products

Product Documents for Calpain 9 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Calpain 9 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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