Calretinin Antibody (S01-2G7)

Novus Biologicals | Catalog # NBP3-19588

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S01-2G7 expressed in HEK293
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Product Specifications

Immunogen

A synthetic peptide of human Calretinin (Uniprot # P22676)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Calretinin Antibody (S01-2G7)

Western Blot: Calretinin Antibody (S01-2G7) [NBP3-19588]

Western Blot: Calretinin Antibody (S01-2G7) [NBP3-19588]

Western Blot: Calretinin Antibody (S01-2G7) [NBP3-19588] - Western blot detection of Calretinin in Rat Brain lysates using NBP3-19588 (1:1000 diluted). Predicted band size: 32kDa. Observed band size: 32kDa.
Calretinin Antibody (S01-2G7)

Immunocytochemistry/Immunofluorescence: Calretinin Antibody (S01-2G7) [NBP3-19588] -

Immunocytochemistry/Immunofluorescence: Calretinin Antibody (S01-2G7) [NBP3-19588] - Immunofluorescence of Calretinin (green) in Hela cells using NBP3-19588 at dilution 1/50, and DAPI (blue)

Applications for Calretinin Antibody (S01-2G7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/20-1/50

Western Blot

1/1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calretinin

Calretinin (CAB29, calbindin 2) is a calcium binding protein that belongs to calbindin family. It is mainly found in neuronal tissue, high expressed in auditory neurons. It has been implicated in as a calcium buffer, calcium sensor, and as a regulator of apoptosis (1). Calretinin is considered as a specific marker of mesothelllal cells; it is the only marker that has the utility in distinguishing between sarcomatoid mesotheliomas and sarcomatoid renal cell carcinomas (2). It is widely used in the panel of makers for distinguishing mesothlioma from adenocarcinoma. Along with calretinin, the panel includes E-cadherin, CEA, and cytokeratin 5/6 (3).

Alternate Names

CAB29, CAL2, CALB2

Gene Symbol

CALB2

Additional Calretinin Products

Product Documents for Calretinin Antibody (S01-2G7)

Certificate of Analysis

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Product Specific Notices for Calretinin Antibody (S01-2G7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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