CASC5 Antibody (HL2158) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25397

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2158 expressed in HEK293

Format

Azide and BSA Free
View all CASC5 Primary Antibodies »

Product Specifications

Immunogen

Synthtic peptide of human CASC5

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CASC5 Antibody (HL2158) - Azide and BSA Free

CASC5 Antibody (HL2158) - Azide and BSA Free

Western Blot: CASC5 Antibody (HL2158) - Azide and BSA Free [NBP3-25397] -

Western Blot: CASC5 Antibody (HL2158) - Azide and BSA Free [NBP3-25397] - A431 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CASC5 antibody [HL2158] (NBP3-25397) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
CASC5 Antibody (HL2158) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: CASC5 Antibody (HL2158) - Azide and BSA Free [NBP3-25397] -

Immunocytochemistry/Immunofluorescence: CASC5 Antibody (HL2158) - Azide and BSA Free [NBP3-25397] - CASC5 antibody [HL2158] detects CASC5 protein at kinetochore by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CASC5 stained by CASC5 antibody [HL2158] (NBP3-25397) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI.

Applications for CASC5 Antibody (HL2158) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CASC5

Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Four known isoforms exist.

Alternate Names

AF15q14, Blinkin, blinkin, bub-linking kinetochore protein, Bub-linking kinetochore protein, cancer susceptibility candidate 5, Cancer susceptibility candidate gene 5 protein, Cancer/testis antigen 29, CT29KIAA1570, D40, hKNL-1, hSpc105, kinetochore null 1 homolog, Kinetochore-null protein 1, KNL1, KNL1ALL1-fused gene from chromosome 15q14 protein, protein CASC5, Protein D40/AF15q14

Gene Symbol

KNL1

Additional CASC5 Products

Product Documents for CASC5 Antibody (HL2158) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CASC5 Antibody (HL2158) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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