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Caspase 3/7 Activity Assay Kit (Colorimetric)
Novus Biologicals | Catalog # NBP3-50230
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Key Product Details
Sample Type & Volume Required Per Well
Cell and tissue samples
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Product Specifications
Kit Type
Activity Assay Kit (Colorimetric)
Species
Non-species specific
Description
Caspase 3, also known as CPP32, Yama or apopain, belongs to the CED-3 subfamily of the caspase family. It is a key enzyme in the process of apoptosis and the most studied caspase in mammalian cells. Caspase 3 exists in the cytoplasm in the form of zymogen under normal condition and has no activity. In the stage of apoptosis, caspase 3 is activated, and the activated caspase 3 consists of two large subunits and two small subunits, which cleaves the corresponding cytoplasmic and nuclear substrates and ultimately leads to apoptosis. This caspase 3/7 activity assay kit is based on caspase 3/7 that can catalyze the substrate Ac-DEVD-pNA to generate yellow pNA (p-Nitroaniline). The pNA has a strong absorption near 405 nm, The activity of caspase 3/7 can be calculated by determining the absorbance at 405 nm.
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Caspase 3/7
Alternate Names
CASP-3, CASP-7, caspase-3, caspase-7, CPP32, CPP32B, ICE-like apoptotic protease 3, SCA-1
Additional Caspase 3/7 Products
Product Documents for Caspase 3/7 Activity Assay Kit (Colorimetric)
Certificate of Analysis
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Product Specific Notices for Caspase 3/7 Activity Assay Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
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