Caspase 3/7 Apoptosis Kit

The Caspase 3/7 Substrate (Green) are based on novel fluorogenic DNA dyes that have been coupled to the caspase 3/7 recognition sequence (DEVD),which is both non-fluorescent and nonfunctional as a DNA dye.When it rapidly crosses cell membranes to enter the cytoplasm and cleaved by caspase 3/7 to form a high-affinity DNA dye that stains the nucleus bright green. Thus, the Caspase 3/7 substrates allow detection of caspase 3/7 activity and visualization of apoptotic nuclear morphology simultaneously. Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-APC, the APC-conjugated format, binds specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy. Caspase 3/7 and Annexin V Double Staining Apoptosis Kit includes Caspase 3/7 Substrates(Green Annexin V-APC Reagent, enables the user to detect two important apoptosis events, caspase 3/7 activation and phosphatidylserine (PS) translocation.