Q: Could I use HeLa cell lysate as a positive control for Caspase 9 primary antibodies in WB?

A: After looking through our testing it does appear you should be fine using HeLa as a positive control cell lysate for Caspase 9 in Western Blot, as we have used this sample successfully in our QC tests and shown expression of Caspase 9.

Caspase-9 Antibody Blocking Peptide

Novus Biologicals | Catalog # NBP1-76961PEP

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
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Key Product Details

Source

Synthetic

Applications

Antibody Competition

View all Caspase-9 Proteins and Enzymes »

Product Specifications

Description

A blocking peptide containing 20 amino acids near the center of human Caspase-9.

Source: Synthetic

(Accession #: P55211)

Application Notes

This peptide is useful as a blocking peptide for NBP1-76961.

For further blocking peptide related protocol, click here.

Protein / Peptide Type

Antibody Blocking Peptide

Formulation, Preparation, and Storage

NBP1-76961PEP

Formulation	PBS pH 7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin.
Preservative	0.02% Sodium Azide
Concentration	0.2 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20C. Avoid freeze-thaw cycles.

Background: Caspase-9

Caspase 9 (CASP9) is a member of the cysteine-aspartic acid protease (caspase) family that is involved in the execution-phase of cell apoptosis. Caspase 9 is expressed as an inactive proenzyme which then undergos proteolytic processing by upstream caspases, to form an active (cleaved) caspase 9 form.

There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Caspase 3 is considered an initiator, as initiated caspase-9 goes on to cleave the effector procaspase-3 and procaspase-7.

Caspase 9 antibodies are important tools for apoptosis studies and research on the caspase family mediated apoptosis signaling pathway.

Alternate Names

CASP9, Caspase9

Gene Symbol

CASP9

Additional Caspase-9 Products

Product Documents for Caspase-9 Antibody Blocking Peptide

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Certificate of Analysis

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Product Specific Notices for Caspase-9 Antibody Blocking Peptide

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Cysteine Proteases and Regulators

Citations for Caspase-9 Antibody Blocking Peptide

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