CCDC8 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85085

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CCDC8 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DNHREEAADNQRAEAPADQGSEVTDNQREEAVHDQRERAPAVQGADNQRAQARAGQRAEAAHNQRAGAPGIQEAEVSAAQG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit CCDC8 Antibody - BSA Free (NBP1-85085) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-CCDC8 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CCDC8 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CCDC8 Antibody [NBP1-85085]

Immunocytochemistry/ Immunofluorescence: CCDC8 Antibody [NBP1-85085]

Immunocytochemistry/Immunofluorescence: CCDC8 Antibody [NBP1-85085] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
Immunohistochemistry-Paraffin: CCDC8 Antibody [NBP1-85085]

Immunohistochemistry-Paraffin: CCDC8 Antibody [NBP1-85085]

Immunohistochemistry-Paraffin: CCDC8 Antibody [NBP1-85085] - Staining of human kidney shows strong cytoplasmic positivity in renal tubules.

Applications for CCDC8 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25 - 2 ug/mL

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. This CCDC8 Antibody is validated for IHC-Fr from a verified customer review.

Reviewed Applications

Read 1 review rated 4 using NBP1-85085 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CCDC8

Alternate Names

coiled-coil domain containing 8, coiled-coil domain-containing protein 8, DKFZp564K0322

Gene Symbol

CCDC8

Additional CCDC8 Products

Product Documents for CCDC8 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CCDC8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CCDC8 Antibody - BSA Free

Customer Reviews for CCDC8 Antibody - BSA Free (1)

4 out of 5
1 Customer Rating
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  • CCDC8 Antibody
    Name: Valeria Di Leo
    Application: Immunohistochemistry-Frozen
    Sample Tested: Skeletal muscle
    Species: Human
    Verified Customer | Posted 11/05/2020
    Human skeletal muscle (quadriceps) stained with anti-CCDC8 (green) and Hoechst (blue).
    Fixative: 4% paraformaldehyde Permeabilization: Methanol gradient (70% for 10 mins, 95% for 10 mins, 100% for 20 mins, 95% for 10 mins, 70% for 10 mins) Protein blocking: 10% NGS diluted in TBST Endogenous biotin blocking: Avidin D solution for 15 mins, Biotin for 15 mins (Vector Laboratories, Inc) Dilution: 1/50 Incubation time: 19 hour(s) and 0 minute(s) · Diluent TBST – NGS 10% Secondary antibody: Life tech/ThermoFisher, A11006, Alexa 488, anti-Rabbit isotype IgG, Host species: Goat, Clonality: Polyclonal
    CCDC8 Antibody - BSA Free NBP1-85085

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Protocols

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FAQs

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