CD21 Antibody (DMC280) - Chimeric

Novus Biologicals | Catalog # NBP3-48307

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # DMC280
View all CD21 Primary Antibodies »

Product Specifications

Immunogen

CD21 recombinant protein expressed in mammalian cells (Accession# P20023). The exact amino acid sequence is proprietary.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Description

This product is shipped at ambient temperature. Store at -20C to -80C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80C (Avoid repeated freezing and thawing).

Scientific Data Images for CD21 Antibody (DMC280) - Chimeric

CD21 Antibody (DMC280) - Chimeric

Western Blot: CD21 Antibody (DMC280) - Chimeric [NBP3-48307]

Anti-CD21 antibody (Catalog Number# NBP3-48307) at 1/1000 dilutionLane : RAJI, whole cell lysateSecondary : Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilutionPredicted band size: 113 kDaObserved band size: 150 kDa
CD21 Antibody (DMC280) - Chimeric

Flow Cytometry: CD21 Antibody (DMC280) - Chimeric [NBP3-48307]

Flow cytometry analysis of antigen binding of anti-human CD21 mAb(NBP3-48307). (A) NBP3-48307 does not bind to Jurkat cells that do not express CD21. (B) A clear peak shift of NBP3-48307 was seen compared to the control when incubated with CD21-expressing Raji cells, indicating strong binding of NBP3-48307 to CD21. Antibodies were incubated at 10 ug/mL.
CD21 Antibody (DMC280) - Chimeric

Flow Cytometry: CD21 Antibody (DMC280) - Chimeric [NBP3-48307]

Flow cytometry analysis with Anti-CD21 (DMC280) on HEK293 cells transfected with human CD21 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram), and Isotype antibody on HEK293 transfected with irrelevant protein (Orange histogram).

Applications for CD21 Antibody (DMC280) - Chimeric

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:1000

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified from cell culture supernatant

Reconstitution

Dissolve the lyophilized antibody in sterile and low endotoxin water. The recommend volume of water is lot specific. Please refer to the instructions printed on the product vial or contact technical support.

Formulation

PBS, pH 7.4, 5-8% Trehalose

Preservative

Trehalose

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in powder form. Store at -80C once reconstituted.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD21

CD21 (complement receptor 2, CR2) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to folicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands.

Alternate Names

C3DR, CD21, CR2

Gene Symbol

CR2

Additional CD21 Products

Product Documents for CD21 Antibody (DMC280) - Chimeric

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CD21 Antibody (DMC280) - Chimeric

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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