CD47 Antibody (050) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-23505

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # 050 expressed in HEK293

Format

Azide and BSA Free
View all CD47 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD47 (Uniprot#: Q61735-1; Met1-Lys140).

Clonality

Monoclonal

Host

Rabbit

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C.

Scientific Data Images for CD47 Antibody (050) - Azide and BSA Free

CD47 Antibody (050)

Western Blot: CD47 Antibody (050) [NBP3-23505] -

Western Blot: CD47 Antibody (050) [NBP3-23505] - NBP3-23505 at 1:500 dilution. Lane A: Mouse brain tissue Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 35 kDa
CD47 Antibody (050)

Immunoprecipitation: CD47 Antibody (050) [NBP3-23505] -

Immunoprecipitation: CD47 Antibody (050) [NBP3-23505] - CD47 was immunoprecipitated using: Lane A: 0.5 mg Mouse brain Whole Cell Lysate 4 uL NBP3-23505 and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: NBP3-23505, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa

Applications for CD47 Antibody (050) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD47

CD47 antigen, also known as integrin associated protein (IAP), has a very broad tissue distribution. CD47 is expressed on all hematopoietic cells, including leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. There are approximately 50.000 CD47 molecules per erythrocyte. The glycoprotein is deficient in erythrocytes of the rare Rh null phenotype. CD47 is a heavily N glycosylated cell membrane glycoprotein of apparent molecular weight 47-52 kDa. It may play a role as a signaltransducer in the regulation of cation fluxes across cell membranes and in the chemotactic and adhesive interactions of leukocytes with endothelial cells.

Alternate Names

CD47, IAP, MER6, OA3

Gene Symbol

CD47

Additional CD47 Products

Product Documents for CD47 Antibody (050) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CD47 Antibody (050) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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Associated Pathways

A-beta Pathways: Uptake & Degradation A-beta Pathways: Uptake & Degradation Thumbnail