Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # 4C8

Format

BSA Free
View all CD52 Primary Antibodies »

Product Specifications

Immunogen

Human lymphocytes

Specificity

The mouse monoclonal antibody 4C8 recognizes CD52, a GPI-anchored glycopeptide expressed on the surface of lymphocytes, macrophages, epithelial cells of male reproductive tract, and mature sperms.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Scientific Data Images for CD52 Antibody (4C8) - BSA Free

Flow Cytometry: CD52 Antibody (4C8) - BSA Free [NBP3-11701]

Flow Cytometry: CD52 Antibody (4C8) - BSA Free [NBP3-11701]

Flow Cytometry: CD52 Antibody (4C8) [NBP3-11701] - Separation of lymphocytes stained anti-human CD52 (4C8) purified antibody (concentration in sample 0,6 ug/ml, GAM APC, red-filled) from lymphocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).
Flow Cytometry: CD52 Antibody (4C8) - BSA Free [NBP3-11701]

Flow Cytometry: CD52 Antibody (4C8) - BSA Free [NBP3-11701]

Flow Cytometry: CD52 Antibody (4C8) [NBP3-11701] - Surface staining pattern of human peripheral whole blood stained using anti-human CD52 (4C8) purified antibody (concentration in sample 0,6 ug/ml, GAM APC).

Applications for CD52 Antibody (4C8) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD52

CD52 (CAMPATH-1, HE5) is a highly glycosylated GPI-anchored 21-28 kDa glycopeptide which is present at high levels on lymphocytes, macrophages, epithelial cells of male reproductive tract and mature sperm. Its 12-amino acid beckbone carries a complex N-linked carbohydrate moiety, which differs between sperm and leukocyte CD52, as well as the GPI anchor does. CD52 can be acquired by sperm cells from seminal plasma, where it is released by epithelial cells. Although CD52 is not an essential T-cell costimulator, its triggering results in activation of normal human T cells. CD52 is a very good target for antibody/complement-mediated cell lysis.

Alternate Names

CAMPATH-1, CD52, CDw52, He5, HEL-S-171mP

Gene Symbol

CD52

Additional CD52 Products

Product Documents for CD52 Antibody (4C8) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD52 Antibody (4C8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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