Key Product Details

Species Reactivity

Chicken

Applications

Flow Cytometry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # EP42
View all CD8 beta Primary Antibodies »

Product Specifications

Immunogen

Chicken splenocytes

Reactivity Notes

Chicken

Specificity

beta chain (Mr 34 kDa) of chicken CD8

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for CD8 beta Antibody (EP42) [FITC]

Flow Cytometry: CD8 beta Antibody (EP42) [FITC] [NBP1-28301] - Chicken IEL were double stained with mouse antichicken CD8beta-FITC and mouse anti-chicken CD8beta-RPE. Small lymphocytes then were gated and analyzed on a FACScan (Trademark) flow cytometer (BDIS, San Jose, CA).

Applications for CD8 beta Antibody (EP42) [FITC]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD8 beta

In the chicken, the CD8 molecule is present in two forms: (i) a homodimer of two alpha chains, and (ii) a heterodimer of an alpha chain and a beta chain. (1) While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8alpha- and CD8beta-chains, a relatively large proportion of the CD8+ TCR-gamma delta cells in the spleens of embryos and young chicks express only the alpha-chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved, but there is a population of TCR-gamma delta CD8alpha beta cells in the chicken that is not found in rodents. (3) Chicken CD8 is expressed on approximately 80% of thymocytes, 45% of blood mononuclear cells and 50% of spleen cells, but 1% of cells in the bursa and bone marrow. (1,2) MAb EP42 recognizes the CD8beta chain. (1)

Alternate Names

CD8 beta, CD8b, CD8B1, Leu2, LY3, LYT3, P37

Gene Symbol

CD8B

Additional CD8 beta Products

Product Documents for CD8 beta Antibody (EP42) [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CD8 beta Antibody (EP42) [FITC]

Protect conjugated forms from light.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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