Key Product Details

Species Reactivity

Validated:

Human, Mouse, Hamster

Cited:

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Armenian Hamster IgG3 Clone # 2F7

Format

Azide and BSA Free
View all CD81 Primary Antibodies »

Product Specifications

Immunogen

Mouse epithelial cell line PAM212

Reactivity Notes

Human reactivty reported in (PMID:23260141).

Specificity

Mouse CD81 (Mr 25 kDa), a member of the transmembrane 4 integral membrane protein family

Clonality

Monoclonal

Host

Armenian Hamster

Isotype

IgG3

Scientific Data Images for CD81 Antibody (2F7) - Azide and BSA Free

Flow Cytometry: CD81 Antibody (2F7) - Azide and BSA Free [NBP1-28136]

Flow Cytometry: CD81 Antibody (2F7) - Azide and BSA Free [NBP1-28136]

Flow Cytometry: CD81 Antibody (2F7) [NBP1-28136] - Chinese hamster ovary cell line CHO-K1 and BALB/cAnN mouse B lymphocyte cell line A20 were stained with Hamster Anti-Mouse CD81-APC.
Flow Cytometry: CD81 Antibody (2F7) - Azide and BSA Free [NBP1-28136]

Flow Cytometry: CD81 Antibody (2F7) - Azide and BSA Free [NBP1-28136]

Flow Cytometry: CD81 Antibody (2F7) [NBP1-28136] - 0.1 ug/1^6 The A20 cell line, a murine B cell lymphoma, was stained with hamster anti-mouse CD81-R: PE or hamster IgG-R: PE isotype control, following which the cells were analyzed for the expression of CD81 on a flow cytometer.
Flow Cytometry: CD81 Antibody (2F7) - Azide and BSA Free [NBP1-28136]

Flow Cytometry: CD81 Antibody (2F7) - Azide and BSA Free [NBP1-28136]

Flow Cytometry: CD81 Antibody (2F7) [NBP1-28136] - Analysis using the FITC conjugate of NBP1-28136. Staining of CHO-K1 cell line (Gray) and BALB/CAnN B lymphocyte cell line A20 (Purple).

Applications for CD81 Antibody (2F7) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.1M BBS (pH 8.2)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD81

CD81/ TAPA-1 is an integral membrane protein expressed on a variety of cell types and has a high degree of sequence homology between human and mouse. CD81 is expressed on thymic stromal cells where it plays an important role in the transition of gamma-delta +T cells to more mature T cells with alpha-beta T cell receptors. Immunohistochemical staining has revealed that its expression is localized to the subcapsular region of the thymus and, specifically, on cells that have distinct clustering patterns. It has been speculated that the ligand for CD81 is the pre-T cell receptor which is composed of a TCR beta chain and glycoprotein pT alpha. The monoclonal antibody 2F7 can block thymocyte interaction with CD81 in vitro.

Alternate Names

CD81, TAPA-1, TSPAN28

Gene Symbol

CD81

Additional CD81 Products

Product Documents for CD81 Antibody (2F7) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CD81 Antibody (2F7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD81 Antibody (2F7) - Azide and BSA Free

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