CD99 Antibody (MEM-131) [PE]

Novus Biologicals | Catalog # NBP1-41282

Clone MEM-131 was used by HLDA to establish CD designation.
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Mouse IgM Clone # MEM-131
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Product Specifications

Immunogen

HPB-ALL human peripheral blood leukemia T-cell line

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species.

Specificity

The antibody MEM-131 reacts with CD99R, an epitope restricted to a subset of CD99 molecule expressed on myeloid cells, NK cells and T lymphocytes. HLDA V; WS Code AS S020 HLDA V; WS Code T T-E2.02 HLDA V; WS Code T T-017

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Description

Store in the dark. Avoid prolonged exposure to light.

Applications for CD99 Antibody (MEM-131) [PE]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 10^6 cells in a suspension.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Size Exclusion Chromatography

Formulation

Tris buffered saline (TBS), pH 8.0

Preservative

15mM Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD99

CD99 (E2, MIC2) is a transmembrane glycoprotein that is involved in regulation of T cell addhesive properties and programmed cell death distinct from typical apoptosis course. CD99 roles are specific to certain stages of T cell differentiation such as corticothymocytes. CD99R isoform expression is restricted in the haematopoietic system to T, NK and myeloid cells.

Alternate Names

CD99, MIC2, pilr-1, PILR-L

Gene Symbol

CD99

Additional CD99 Products

Product Documents for CD99 Antibody (MEM-131) [PE]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CD99 Antibody (MEM-131) [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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