Cdc23 Antibody (1Q2I3)

Name: Cdc23 Antibody (1Q2I3)
Brand: Novus Biologicals
SKU: NBP3-16631
Price: 389 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16631

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1Q2I3 expressed in HEK293

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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 498-597 of human CDC23 (Q9UJX2). YSCGEIVEHLEESTAFRYLAQYYFKCKLWDEASTCAQKCCAFNDTREEGKALLRQILQLRNQGETPTTEVPAPFFLPASLSANNTPTRRVSPLNLSSVTP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cdc23 Antibody (1Q2I3)

Western Blot: Cdc23 Antibody (1Q2I3) [NBP3-16631]

Western Blot: Cdc23 Antibody (1Q2I3) [NBP3-16631] - Western blot analysis of extracts of various cell lines, using Cdc23 Rabbit mAb (NBP3-16631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunocytochemistry/ Immunofluorescence: Cdc23 Antibody (1Q2I3) [NBP3-16631]

Immunocytochemistry/Immunofluorescence: Cdc23 Antibody (1Q2I3) [NBP3-16631] - Immunofluorescence analysis of HeLa cells using Cdc23 Rabbit mAb (NBP3-16631) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: Cdc23 Antibody (1Q2I3) [NBP3-16631] -

Immunocytochemistry/ Immunofluorescence: Cdc23 Antibody (1Q2I3) [NBP3-16631] - Immunofluorescence analysis of HeLa cells using Cdc23 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for Cdc23 Antibody (1Q2I3)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cdc23

CDC23/APC8 is a component of the APC/c (anaphase promoting complex/cyclosome) which is responsible for the ubiquitination and degradation of securin and cyclin B that prompts the onset of anaphase and exit from mitosis. The APC/c is composed of at least 11 subunits. Three subunits, CDC27 CDC16 and CDC23, contain a TPR (tetratricopeptide repeat) important for protein-protein interactions.

Alternate Names

ANAPC8anaphase promoting complex subunit 8, Anaphase-promoting complex subunit 8, APC8CDC23 (cell division cycle 23, yeast, homolog), cell division cycle 23 homolog (S. cerevisiae), cell division cycle protein 23 homolog, CUT23, Cyclosome subunit 8

Gene Symbol

CDC23

Additional Cdc23 Products

Product Documents for Cdc23 Antibody (1Q2I3)

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Certificate of Analysis

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Product Specific Notices for Cdc23 Antibody (1Q2I3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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