Recombinant Human CDC25A His Protein

Novus Biologicals | Catalog # NBP1-51019

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE
View all CDC25A Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-524 of Human CDC25A

Source: E.coli

Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMELG PEPPHRRRLL FACSPPPASQ PVVKALFGAS AAGGLSPVTN LTVTMDQLQG LGSDYEQPLE VKNNSNLQRM GSSESTDSGF CLDSPGPLDS KENLENPMRR IHSLPQKLLG CSPALKRSHS DSLDHDIFQL IDPDENKENE AFEFKKPVRP VSRGCLHSHG LQEGKDLFTQ RQNSAPARML SSNERDSSEP GNFIPLFTPQ SPVTATLSDE DDGFVDLLDG ENLKNEEETP SCMASLWTAP LVMRTTNLDN RCKLFDSPSL CSSSTRSVLK RPERSQEESP PGSTKRRKSM SGASPKESTN PEKAHETLHQ SLSLASSPKG TIENILDNDP RDLIGDFSKG YLFHTVAGKH QDLKYISPEI MASVLNGKFA NLIKEFVIID CRYPYEYEGG HIKGAVNLHM EEEVEDFLLK KPIVPTDGKR VIVVFHCEFS SERGPRMCRY VRERDRLGNE YPKLHYPELY VLKGGYKEFF MKCQSYCEPP SYRPMHHEDF KEDLKKFRTK SRTWAGEKSK REMYSRLKKL

Purity

>80%, by SDS-PAGE

Predicted Molecular Mass

63.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CDC25A His Protein

SDS-PAGE: Recombinant Human CDC25A His Protein [NBP1-51019]

SDS-PAGE: Recombinant Human CDC25A His Protein [NBP1-51019]

SDS-Page: Recombinant Human CDC25A Protein [NBP1-51019] - 15 % SDS-PAGE (3ug)

Formulation, Preparation, and Storage

NBP1-51019
Formulation 20 mM Tris-HCl buffer (pH 8.0), 5mM DTT, 20% glycerol, 0.2 M NaCl, 1 mM EDTA
Preservative No Preservative
Concentration 0.25 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDC25A

M-phase inducer phosphatase 1, also known as CDC25A, is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Recombinant human CDC25A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Cell Division Cycle 25A

Alternate Names

CDC25A2, CDC25A2-CAG isoform, cell division cycle 25 homolog A (S. cerevisiae), cell division cycle 25 homolog A (S. pombe), cell division cycle 25A, Dual specificity phosphatase Cdc25A, EC 3.1.3.48, M-phase inducer phosphatase 1

Gene Symbol

CDC25A

Additional CDC25A Products

Product Documents for Recombinant Human CDC25A His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human CDC25A His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle
Phosphatases and Regulators

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