Recombinant Human CDC42 T7 Protein

Novus Biologicals | Catalog # NBP1-30243

Novus Biologicals
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Key Product Details

Source

E. coli

Accession Number

P60953

Tag

T7

Applications

SDS-PAGE
View all CDC42 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal T7-tag and corresponding to the amino acids 1- 188 of Human CDC42

Source: E.coli

Amino Acid Sequence: MASMTGGQQM GRGSHMQTIK CVVVGDGAVG KTCLLISYTT NKFPSEYVPT VFDNYAVTVM IGGEPYTLGL FDTAGQEDYD RLRPLSYPQT DVFLVCFSVV SPSSFENVKE KWVPEITHHC PKTPFLLVGT QIDLRDDPST IEKLAKNKQK PITPETAEKL ARDLKAVKYV ECSALTQKGL KNVFDEAILA ALEPPEPKKS RRC

Purity

>95%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

22.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CDC42 T7 Protein

SDS-PAGE: Recombinant Human CDC42 T7 Protein [NBP1-30243]

SDS-PAGE: Recombinant Human CDC42 T7 Protein [NBP1-30243]

SDS-Page: Recombinant Human CDC42 Protein [NBP1-30243] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Formulation, Preparation, and Storage

NBP1-30243
Formulation 20 mM Tris-HCl buffer (pH8.0), 1 mM DTT, 10% glycerol, 2 mM EDTA
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDC42

Cell division cycle 42 isoform 1, also known as CDC42, is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. Also, this protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Loss of CDC42 function caused an increase in the endocytotic uptake of apical proteins, including apical polarity factors such as Crumbs. Recombinant human CDC42, fused to T7-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Cell Division Cycle 42

Alternate Names

G25K

Gene Symbol

CDC42

UniProt

P60953 (Human)

Additional CDC42 Products

Product Documents for Recombinant Human CDC42 T7 Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human CDC42 T7 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle

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