CDT2 Antibody

Novus Biologicals | Catalog # NB100-40840

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot, Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all CDT2 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 680 and the C-terminus (residue 730) of human Denticleless Homolog (Chromatin Licensing and DNA Replicating Factor 2) using the numbering given in entry NP_057532.1 (GeneID 51514)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CDT2 Antibody

Western Blot: CDT2 Antibody [NB100-40840]

Western Blot: CDT2 Antibody [NB100-40840]

Western Blot: CDT2 Antibody [NB100-40840] - Whole cell lysate (15 ug) from Jurkat, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DTL/CDT2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Immunohistochemistry-Paraffin: CDT2 Antibody [NB100-40840]

Immunohistochemistry-Paraffin: CDT2 Antibody [NB100-40840]

Immunohistochemistry-Paraffin: CDT2 Antibody [NB100-40840] - Section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-DTL/CDT2 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: CDT2 Antibody [NB100-40840]

Immunohistochemistry-Paraffin: CDT2 Antibody [NB100-40840]

Immunohistochemistry-Paraffin: CDT2 Antibody [NB100-40840] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-DTL/CDT2 used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB
Immunoprecipitation: CDT2 Antibody [NB100-40840]

Immunoprecipitation: CDT2 Antibody [NB100-40840]

Immunoprecipitation: CDT2 Antibody [NB100-40840] - Detection of human DTL/CDT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DTL/CDT2 antibody NB100-40840 (lot NB100-40840-3) used for IP at 6 ug per reaction. DTL/CDT2 was also immunoprecipitated by a previous lot of this antibody (lot NB100-40840-2). For blotting immunoprecipitated DTL/CDT2, NB100-40840 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
CDT2 Antibody

Immunohistochemistry: CDT2 Antibody [NB100-40840] -

Expression profiles of DTL in 7 GC cell linesA. Quantitative real-time RT–PCR and western blotting analysis were performed using a DTL-specific antibody to determine DTL mRNA (top) and protein (bottom) expression in the gastric cancer cell lines KatoIII, NUGC4, MKN7, HGC27, MKN28, MKN45, and MKN74. DTL overexpression was observed in the KatoIII, NUGC4, HGC27, and MKN28 cells (4/7 lines, 57%). B. A formalin-fixed gastric cancer NUGC4 cell line that overexpresses DTL, in which > 50% of cells stained positively, was used as a positive control; an MKN45 cell line with a low expression of DTL was included as a negative control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26472028), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for CDT2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 -1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.

Reviewed Applications

Read 1 review rated 4 using NB100-40840 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CDT2

DTL promotes genomic stability through two distinct mechanisms. First, it is an essential component of the CUL4-DDB1 complex that controls CDT1 levels, thereby preventing rereplication. Second, it is required for the early G2/M checkpoint.

Alternate Names

CDT2RA-regulated nuclear matrix-associated protein, CDW1, DCAF2DDB1 and CUL4 associated factor 2, DDB1- and CUL4-associated factor 2, denticleless homolog (Drosophila), L2DTLRA regulated nuclear matrix associated protein, Lethal(2) denticleless protein homolog, RAMPdenticleless protein homolog, Retinoic acid-regulated nuclear matrix-associated protein

Entrez Gene IDs

51514 (Human)

Gene Symbol

DTL

UniProt

Q9NZJ0 (Human)

Additional CDT2 Products

Product Documents for CDT2 Antibody

Certificate of Analysis

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Product Specific Notices for CDT2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for CDT2 Antibody (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23478441
    Species: Human
    Verified Customer | Posted 12/12/2014

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Protocols

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